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ISC Class XII Board Exam 2018 : Biotechnology Paper 1 (Theory)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi

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BIOTECHNOLOGY PAPER 1 (THEORY) (Maximum Marks: 70) (Time allowed: Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.) --------------------------------------------------------------------------------------------------------------------Answer Question 1 (compulsory) from Part I and five questions from Part II. The intended marks for questions or parts of questions are given in brackets [ ]. --------------------------------------------------------------------------------------------------------------------PART I (20 Marks) Answer all questions. Question 1 (a) Mention any one significant difference between each of the following: (i) (ii) (iii) (iv) (v) [5] Plasmids and cosmids Nucleotide and Nucleoside Lagging strand and leading strand Multipotent cells and unipotent cells Microinjection and biolistic (b) Answer the following questions: (i) Who coined the term vitamin? Write the chemical name of vitamin D. (ii) Why is amino acid said to be amphoteric? (iii) What is Bioremediation? (iv) What is a primer? (v) What are cryoprotectants? [5] (c) Write the full form of each of the following: [5] (i) NBPGR (ii) ARS (iii) RFLP (iv) HEPA (v) SCP --------------------------------------------------------------------------------------------------------------------This paper consists of 4 printed pages. 1218-878A Copyright reserved. Turn over (d) Explain briefly: [5] (i) Gene splicing (ii) Supramolecular assembly (iii) Interferon (iv) Gene scan (v) Saponification PART II (50 Marks) Answer any five questions. Question 2 (a) Explain in detail, how Dolly, the sheep was created. [4] (b) Mention any two chemical properties of each of the following: [4] (c) (i) Proteins (ii) Carbohydrates What are Okazaki fragments? How are they joined? [2] Question 3 (a) Describe the effect of each of the following factors on enzyme activity: (i) pH (ii) Temperature [4] (iii) Enzyme concentration (iv) (b) (c) Concentration of products With reference to suspension culture, explain the following: (i) A chemostat (ii) A turbidostat What is genomics? What are its different types? [4] [2] Question 4 (a) What are the basic facilities that should be available for tissue culture in a biotechnology laboratory? [4] (b) Explain the experiment which proves the semi-conservative mode of replication. [4] (c) What is cDNA? [2] --------------------------------------------------------------------------------------------------------------------2 1218-878A Question 5 (a) Explain any four methods employed to induce haploid production. [4] (b) Describe the automated method of DNA sequencing. [4] (c) What is the difference between gel electrophoresis and gel permeation. [2] Question 6 (a) What is in vitro pollination? Why is it done? Write the steps involved in this process. [4] (b) Why is Agrobacterium called a natural genetic engineer? How does it help in creating transgenic plants? [4] (c) Write a short note on site directed mutagenesis. [2] Question 7 (a) What is HGP? Name any two scientists involved in this. Write any two achievements of HGP. [4] (b) List the functions of the following in Bioinformatics: [4] (c) (i) ENTREZ (ii) PDB (iii) FASTA (iv) MGD Mention two differences between the organisation of prokaryotic and eukaryotic genomes. [2] Question 8 (a) Briefly describe the steps involved in the Southern blotting technique. [4] (b) What is the need of germplasm conservation? Give an account of the in-situ and [4] ex-situ conservation of germplasm. (c) What is peptidoglycan? Where is it found? [2] --------------------------------------------------------------------------------------------------------------------3 1218-878A Turn over Question 9 (a) (b) (c) How are biomolecules separated by the following techniques: (i) Ion exchange chromatography. (ii) Partition chromatography. What is the cause and the symptoms of the following diseases: (i) Sickle cell anaemia (ii) Alkaptonuria What is the difference between peptide bond and phosphodiester bond? [4] [4] [2] --------------------------------------------------------------------------------------------------------------------4 1218-878A

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