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ISC Class XII Board Exam 2019 : Biotechnology Paper 2 (Practical)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi
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BIOTECHNOLOGY PAPER 2 (PRACTICAL) (M*vimum Marks:30) (Time allowed: Three hours) (Candidates' are alktwed additionul I5 minutes _for only reading the paper. They must NOT start writing during this time.) Answer ctll questions. The intended marks.for questions or parts ctf'questions are given in bruckets Question (a) [ /. I You are provided with an explant (a small piece of leafl labelled E, a Petri plate labelled G and metallic forceps labelled M. Using appropriate method(s), sterilise each of these samples. You may use autoclave / hot air oven / chemicals provided to you, as required. Answer the following questions: (i) Which sample / samples can be sterilised by applying dry heat as well as wet sterilisation method? (ii) Which sample (iii) (b) / samples cannot be sterilisecl sterilisation method? Give a reason. heat tU by applying dry heat or wet heat I1l Name the method by which, the sample / samples mentioned by you in (ii) above, be sterilised. can tU You are provided with a Petri plate labelled L. containing bacterial colonies isolated from curd. Using this sample, perform the follorving experiment: Pick up a bacterial colony with the help of a needle clean glass slide and make a thin smear. / inoculation loop and spread it on a Next. add a few drops of crystal violet stain to the smear and spread it evenly on the slide. Wait for 30 seconds. Add a few drops of iodine solution to the smear and keep it for 2 minutes. Rinse the smear r,vith distilled water to remove extra stain. Ncw, wash the smear with ethanol. This paper consists of 3 printed pages and t2t9-8788 @Copyrighl reserved. I blank page. Turn over

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