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ISC Class XII Board Exam 2017 : Biotechnology Paper 1 (Theory)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi

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BIOTECHNOLOGY PAPER 1 (THEORY) (Maximum Marks: 70) (Time allowed: Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.) --------------------------------------------------------------------------------------------------------------------Answer Question 1 (compulsory) from Part I and five questions from Part II. The intended marks for questions or parts of questions are given in brackets [ ]. --------------------------------------------------------------------------------------------------------------------PART I (20 Marks) Answer all questions. Question 1 (a) Mention any one significant difference between each of the following: (i) (ii) (iii) (iv) (v) [5] Reducing sugar and non-reducing sugar. Triploids and haploids. Lac operon and Trp operon Blunt end and sticky end. Spectroscopy and colorimetry. (b) Answer the following questions: (i) Who developed the microbe called super bug, which was designed to degrade spilled oil? (ii) Name any two growth regulators used in a culture medium. (iii) What is an apoenzyme? (iv) How is the disease albinism caused? (v) State any one limitation of gynogenesis. [5] (c) Write the full form of each of the following: [5] (i) AFLP (ii) SSBs (iii) BAC (iv) CIMAP (v) PAGE --------------------------------------------------------------------------------------------------------------------This paper consists of 4 printed pages. 1217-878 A Copyright reserved. Turn over (d) Explain briefly: [5] (i) Polyadenylation (ii) Lock and key model of enzyme action (iii) Edible vaccine (iv) Vascular differentiation (v) Seedless crops PART II (50 Marks) Answer any five questions. Question 2 (a) Briefly explain the structure of tRNA. Write its function in protein synthesis. [4] (b) With reference to lipids, explain its: [4] (c) (i) Building blocks. (ii) Any two chemical properties. What is a DNA probe? [2] Question 3 (a) Explain the process involved in the transcription of DNA to mRNA. [4] (b) What are stem cells? Explain the various types of stem cells. [4] (c) Name any two chemicals used to determine the amino acid sequence in protein. [2] Question 4 (a) Explain the following methods of selection of recombinant cells: (i) Insertional inactivation. (ii) Blue white colony [4] (b) Enumerate the steps involved in regenerating a plant from a single cell. [4] (c) What is wobble effect? [2] --------------------------------------------------------------------------------------------------------------------2 1217-878 A Question 5 (a) Discuss the working of PCR technique in detail. [4] (b) Explain the principle and any two applications of each of the following biochemical techniques: (i) Iso electric focussing. (ii) Centrifugation. Where do we find the following carbohydrates: [4] (c) (i) Glycogen (ii) Chitin [2] Question 6 (a) Describe the procedure of sequencing of DNA by Sanger s method. [4] (b) Explain any two physical and any two chemical methods used to synchronize suspension cultures. [4] (c) Name any two industrial enzymes and give their uses. [2] Question 7 (a) Briefly explain the essential features of a vector. [4] (b) What is the principle of cryopreservation? Mention the steps of cryopreservation. [4] (c) What is the importance of pH and solidifying agents in cell cultures? [2] Question 8 (a) (b) (c) Explain how DNA technology has been used to create the following: (i) Tomatoes with delayed ripening. (ii) Bt crops (iii) Virus free crops (iv) Biodegradable plastic List the functions of the following bioinformatics tools: (i) GENSCAN (ii) ENTREZ (iii) FASTA (iv) PIR Name any two media used in plant tissue culture. [4] [4] [2] --------------------------------------------------------------------------------------------------------------------3 1217-878 A Turn over Question 9 (a) What are restriction enzymes? How do they work? What are the different types of [4] restriction enzymes? (b) Define the term proteomics. Explain the various types of proteomics. [4] (c) Differentiate between the following: [2] (i) Local alignment and Global alignment. (ii) EST and STS. --------------------------------------------------------------------------------------------------------------------4 1217-878 A

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