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ISC Class XII Board Exam 2019 : Biotechnology Paper 1 (Theory)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi

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BIOTECHNOLOGY PAPER 1 (THEORY) (Maximum Marks: 70) (Time allowed: Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.) --------------------------------------------------------------------------------------------------------------------Answer Question 1 (compulsory) from Part I and five questions from Part II. The intended marks for questions or parts of questions are given in brackets [ ]. --------------------------------------------------------------------------------------------------------------------PART I (20 Marks) Answer all questions. Question 1 (a) Mention any one significant difference between each of the following: (i) (ii) [5] Anticodon and codon Intrinsic fluorescence and extrinsic fluorescence (iii) Introns and Exons (iv) Genomic DNA library and cDNA library (v) RAM and ROM (b) Answer the following questions: Which amino acid is optically inactive and why? (i) (ii) What is meant by exponential phase? (iii) What are designer oils? (iv) (v) (c) [5] What is palindromic sequence? Which substance is used in diploidization of haploid plants? Write the full form of each of the following: (i) NBRI (ii) NBTB [5] (iii) BLAST (iv) PIR (v) YAC --------------------------------------------------------------------------------------------------------------------This paper consists of 4 printed pages. 1219-878A Copyright reserved. Turn over (d) Explain briefly the following terms: (i) Callus (ii) SNPs [5] (iii) Lyophilisation (iv) Gene cloning (v) Cybrids PART II (50 Marks) Answer any five questions. Question 2 (a) With reference to composition of culture medium, answer the following: (i) Cytokinins (ii) Auxins [4] (b) Explain the induced fit hypothesis of enzyme action with the help of suitable illustrations. [4] (c) Write a note on quaternary structure of proteins. [2] Question 3 (a) Explain the important postulates of central dogma. [4] (b) Name and explain the method used to sterilize the following: [4] (i) Vitamins (ii) Forceps and Scalpels (iii) Nutrient Media (iv) Explant (c) What is the Chargaff s rule of equivalence? [2] Question 4 (a) Differentiate between oils and fats. Discuss hydrolysis, rancidity and hardening shown by lipids. [4] (b) Using tissue culture method one can produce disease free plants. Discuss the method used to produce virus free plants. [4] (c) Write the main objectives of HGP. [2] --------------------------------------------------------------------------------------------------------------------2 1219-878A Question 5 (a) Discuss the mechanism of lac operon model of regulation of gene expression. [4] (b) Give four points of difference between southern blotting technique and northern blotting technique. [4] (c) Give four characteristics of genetic code. [2] Question 6 (a) (b) (c) With reference to vectorless methods of gene transfer explain each of the following: (i) Liposome mediated gene transfer (ii) Electroporation (iii) Transfection (iv) Transformation With reference to application of tissue culture techniques, explain the following: (i) Haploid production (ii) Triploid production What is meant by DNA probe? [4] [4] [2] Question 7 (a) (b) (c) Explain how biotechnology helps in developing following traits in crops: (i) Biodegradable plastic (ii) Pest resistance (iii) Drought resistance (iv) Salinity resistance Write the principle and applications of the following techniques: (i) Hydrophobic interaction (ii) Colorimetry What are start and stop codons? [4] [4] [2] --------------------------------------------------------------------------------------------------------------------3 1219-878A Turn over Question 8 (a) List any four responsibilities carried out by NCBI. (b) Give a comparative account of cell differentiation, dedifferentiation, redifferentiation [4] and vascular differentiation. (c) What is the difference between dNTP and ddNTP? [4] [2] Question 9 (a) Proteins have many important functions in an organism. Justify the statement giving [4] its various roles with an example of each. (b) With reference to screening strategies, explain the following: (c) (i) Insertional Inactivation method (ii) Blue White method How was insulin obtained before the advent of rDNA technology? [4] [2] --------------------------------------------------------------------------------------------------------------------4 1219-878A

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