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ISC Class XII Board Exam 2018 : Biotechnology Paper 2 (Practical)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi
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BIOTECHNOLOGY PAPER 2 (PRACTICAL) (Maximum Marks: 30) (Time allowed: Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.) --------------------------------------------------------------------------------------------------------------------Answer all questions. The intended marks for questions or parts of questions are given in brackets [ ]. -------------------------------------------------------------------------------------------------------------------Question 1 (a) You are provided with a milk sample, labelled M. Take 25 ml of the sample M in a 100 ml beaker and add 25 ml of distilled water to it. Warm the sample in a water bath set at 37oC for 5 minutes. Remove the beaker from the water bath and record the pH of the sample using pH paper. Next, add 0 4N HCl to the beaker drop by drop and stir the mixture continuously until the milk begins to precipitate. Note the pH at which maximum precipitation of milk sample occurs. Filter the precipitate, using muslin cloth. Answer the following questions: (i) Report the initial pH of the milk sample at 37oC and the pH at which maximum [1] precipitation of the milk sample occurs. (ii) Name the reducing sugar present in the above sample. Also, name the precipitate [1] obtained in the experiment. (iii) Explain the basic principle involved in the above experiment. (b) You are given two solutions, labelled A (0 2 M boric acid) and labelled B (0 2 M sodium borate). Take two beakers (250 ml each) and label them X and Y. Pour 50 ml of solution A into both of these beakers. Perform the given experiment and answer the questions that follow. To beaker X, add 2 ml of solution B. Make the volume to 200 ml by adding distilled water and mix it thoroughly. Note the pH of the solution by using pH paper. --------------------------------------------------------------------------------------------------------------------This paper consists of 3 printed pages and 1 blank page. 1218-878B Turn over Copyright reserved. [1]

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