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ISC Class XII Board Exam 2020 : Biotechnology Paper 1 (Theory)

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi

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BIOTECHNOLOGY PAPER 1 (THEORY) (Maximum Marks: 70) (Time allowed: Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.) ---------------------------------------------------------------------------------------------------------------Answer Question 1 (compulsory) from Part I and five questions from Part II. The intended marks for questions or parts of questions are given in brackets [ ]. ---------------------------------------------------------------------------------------------------------------PART I (20 Marks) Answer all questions. Question 1 (a) Mention any one significant difference between each of the following: (i) Nucleotide and Nucleoside (ii) Gene and Genome [5] (iii) Finite cell lines and Continuous cell lines (b) (iv) Primer and Primase (v) Micronutrients and Macronutrients Answer the following questions: (i) What are Polylinker sites? (ii) What is Subtilisin? [5] (iii) Name two types of phage vectors. (c) (iv) What is the role of Agrobacterium tumefaciens in genetic engineering? (v) Why is DNA replication called semi-discontinuous? Write the full form of each of the following: (i) DDBJ (ii) HEPA [5] (iii) RFLP (iv) VNTR (v) MS Medium ---------------------------------------------------------------------------------------------------------------This paper consists of 3 printed pages and 1 blank page. 1220-878A Copyright reserved. Turn over (d) Explain briefly the following: (i) Reverse transcription (ii) Electroporation (iii) Biolistic (iv) Synthetic seeds (v) Flavr savr tomatoes [5] PART II (50 Marks) Answer any five questions. Question 2 (a) (b) (c) Describe the double helical structure of DNA. Mention two differences between RNA and DNA. List the role of the following in protein synthesis: (i) mRNA (ii) rRNA (iii) tRNA (iv) Ribosomes Why are cDNA libraries preferred over genomic libraries? [4] [4] [2] Question 3 (a) (b) (c) Differentiate between each of the following: (i) Blunt end and Sticky end (ii) Hybrid and Cybrid Discuss the role of Biotechnology in making the following: (i) Humulin (ii) Hepatitis B vaccine What is FBS? What is its role in animal cell culture? [4] [4] [2] Question 4 (a) (b) (c) What is SCP? Describe the advantages and disadvantages of SCP. Explain the role of any four enzymes in the process of DNA replication. What is micropropagation? Write its use. [4] [4] [2] Question 5 (a) (b) (c) Draw a neat and labelled diagram of a bioreactor. How did Messelson and Stahl prove the semi conservative mode of replication? What is the use of haemocytometer? [4] [4] [2] ---------------------------------------------------------------------------------------------------------------2 1220-878A Question 6 (a) Give four points of difference between inducible operon and repressible operon. [4] (b) Write short notes on the following: [4] (c) (i) Western blotting (ii) Tissue engineering What is embryo rescue? [2] Question 7 (a) Explain the role of the following in biotechnology: (i) Thermus aquaticus (ii) Bacillus thuringiensis (iii) Escherichia coli (iv) CaMV [4] (b) Explain the methodology involved in the creation of the first mammalian clone, Dolly. [4] (c) What is Golden rice? Why is it considered to be nutritionally superior to the normal rice? [2] Question 8 (a) Enumerate the steps involved in the extraction and purification of DNA from bacterial cell. [4] (b) Write short notes on the following: [4] (c) (i) EMBL (ii) SWISS-PROT Write the difference between defined media and differential media. [2] Question 9 (a) What is somatic hybridization? Explain the steps involved in this technique with the help of an example. [4] (b) What is genomics? What are the basic criteria in selecting the organism for its genome sequencing? Write the names of any two types of DNA used for sequencing. [4] (c) Write a short note on Taxonomy Browser. [2] ---------------------------------------------------------------------------------------------------------------3 1220-878A Turn over

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