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GATE 2018 : Life Sciences [Section Q / Biochemistry] (with Final Answer Keys)

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GATE 2018 Biochemistry-XL(Q) Q. 1 Q. 10 carry one mark each & Q.11 - Q.20 carry two marks each. Q.1 To which one of the following classes of enzymes does chymotrypsin belong? (A) Oxidoreductase (B) Hydrolase (D) Isomerase The substrate saturation profile of an enzyme that follows Michaelis-Menten kinetics is depicted in the figure. What is the order of the reaction in the concentration range between 0.8 to 1.4 M? Initial velocity (M. min-1) Q.2 (C) Transferase 30 25 20 15 10 5 0.2 0.4 0.6 0.8 1.0 1.2 1.4 [S] (M) (A) Zero Q.3 (B) Fraction (B) Half Chair (B) (C) (D) v v v v [S] [S] [S] Which one of the following amino acids is responsible for the intrinsic fluorescence of proteins? (B) Met (C) His (D) Trp The glycosylation of the proteins occurs in_____. (A) glyoxysomes (C) Golgi apparatus XL(Q) (D) Planar (A) (A) Pro Q.6 (C) Chair Which one of the following profiles represent the phenomenon of cooperativity? [S] Q.5 (D) Second Which one of the following conformations of glucose is most stable? (A) Boat Q.4 (C) First (B) lysosomes (D) plasma membrane 1/4 GATE 2018 Q.7 Biochemistry-XL(Q) Which one of the following properties of the myeloma cells is used in the hybridoma technology to generate monoclonal antibody? (A) lack of thymidylate synthase (B) over-expression of hypoxanthine-guanine phosphoribosyl transferase (C) over-expression of inosine 5 -monophosphate cyclohydrolase (D) lack of hypoxanthine-guanine phosphoribosyl transferase Q.8 The movement of protons through the FoF1-ATPase during mitochondrial respiration is required for ____ (A) the increase in pH of mitochondrial matrix. (B) changing the conformation of FoF1-ATPase to expel the ATP. (C) importing Pi from inter membrane space. (D) decreasing the affinity of ADP to FoF1-ATPase. Q.9 The number of NADP+ molecules required to completely oxidize one molecule of glucose to CO2 through pentose phosphate pathway is ____ (correct to integer number). Q.10 Measurement of the absorbance of a solution containing NADH in a path length of 1cm cuvette at 340 nm shows the value of 0.31. The molar extinction coefficient of NADH is 6200 M-1 cm-1. The concentration of NADH in the solution is ___ M (correct to integer number). Q. 11 Q. 20 carry two marks each. Q.11 Among the reagents given below which one of the combination of reagents will NOT break the disulphide bonds in the immunoglobulin molecules? (P) Reduced glutathione (R) Sodium dodecyl sulphate (A) R&S (B) P&R (Q) Dithiothritol (S) Methionine (C) P&S (D) Q&R Q.12 Match the protein elution condition given in Group I with the appropriate chromatography matrices from Group II. P Q R S Group I Increasing concentration of sodium chloride Increasing concentration of histidine Decreasing concentration of ammonium sulphate Decreasing concentration of H+ (A) P-iii; Q-iv; R-i; S-ii (C) P-i; Q-ii; R-iii; S-iv XL(Q) i ii iii iv Group II Phenyl-Sepharose Chromatofocusing DEAE-Sephacryl Ni-NTA (B) P-ii; Q-iv; R-i; S-iii (D) P- iv; Q-ii; R-iii; S-i 2/4 GATE 2018 Biochemistry-XL(Q) Q.13 Which one of the following is NOT a neurotransmitter? (A) Adrenaline (B) Glutamate (C) Histamine (D) Histidine Q.14 The type-II hypersensitivity reaction is mainly mediated by____. (A) IgE (B) IgM (C) IgA (D) T cells Q.15 Which one the following reaction mechanisms drives the conversion of low energy 3phosphoglyceraldehyde to high energy 1,3-bisphosphoglycerate? (A) Oxidation without anhydride bond formation (B) Oxidation coupled with anhydride bond formation (C) Substrate level phosphorylation (D) Formation of carboxylate Q.16 A polymerase reaction is carried out for 10 cycles in a volume of 1 ml with 5 molecules of template DNA. Assuming that the efficiency of the reaction is 100 %, the number of molecules of DNA present in 100 l at the end of the reaction is ___ (correct to integer number). Q.17 The secondary structure topology diagram of 400 amino acid long Protein-X is depicted in the figure. The start and end amino acid residue numbers of each -helix are marked. The percentage (correct to integer number) of residues forming -helix is____. N 1 21 70 -helix 225 249 -helix 320 324 -helix 400 C Q.18 An enzyme follows Michaelis-Menten kinetics with substrate S. The fraction of the maximum velocity (Vmax) will be observed with the substrate concentration [S] = 4Km is _____ (correct to one decimal place). (Km is Michaelis-Menten constant) Q.19 The mass spectrum of benzoic acid will generate the fragment as a base peak (100% relative abundance) of m/z (mass to charge ratio) at ____ (correct to integer number). XL(Q) 3/4 GATE 2018 Biochemistry-XL(Q) Q.20 The standard free energy G ) values of reactions catalyzed by citrate lyase and citrate synthetase are -670 and -8192 cal/mol, respectively. Citrate Citrate lyase Acetate + Oxaloacetate G1 = -670 cal/mole Acetyl-CoA + Oxaloacetate + H2O Citrate synthetase Citrate + CoA G2 = -8192 cal/mole The standard free energy (in cal/mol) of acetyl-CoA hydrolysis is ___ (correct to integer number). END OF THE QUESTION PAPER XL(Q) 4/4 Q.No. Type Section Key/Range Marks 1 MCQ GA A 1 2 MCQ GA C 1 3 MCQ GA B 1 4 MCQ GA B 1 5 MCQ GA B 1 6 MCQ GA A 2 7 MCQ GA D 2 8 MCQ GA D 2 9 MCQ GA B 2 10 MCQ GA C 2 1 MCQ XL P A 1 2 MCQ XL P D 1 3 MCQ XL P D 1 4 NAT XL P 11 to 11 1 5 NAT XL P 4 to 4 1 6 MCQ XL P D 2 7 MCQ XL P D 2 8 MCQ XL P A 2 9 MCQ XL P D 2 10 MCQ XL P A 2 11 MCQ XL P C 2 12 MCQ XL P B 2 13 NAT XL P 1.39 to 1.43 2 Q.No. Type Section Key/Range Marks 14 NAT XL P 7.39 to 7.54 2 15 NAT XL P 13.40 to 13.36 2 1 MCQ XL Q B 1 2 MCQ XL Q A 1 3 MCQ XL Q C 1 4 MCQ XL Q C 1 5 MCQ XL Q D 1 6 MCQ XL Q C 1 7 MCQ XL Q D 1 8 MCQ XL Q B 1 9 NAT XL Q 12 to 12 1 10 NAT XL Q 50 to 50 1 11 MCQ XL Q A 2 12 MCQ XL Q A 2 13 MCQ XL Q D 2 14 MCQ XL Q B 2 15 MCQ XL Q B 2 16 NAT XL Q 512 to 512 2 17 NAT XL Q 20 to 20 2 18 NAT XL Q 0.8 to 0.8 2 19 NAT XL Q 77 to 77 2 20 NAT XL Q 8862 to 8862 2 1 MCQ XL R A 1 Q.No. Type Section Key/Range Marks 2 MCQ XL R B 1 3 MCQ XL R C 1 4 MCQ XL R D 1 5 MCQ XL R B 1 6 MCQ XL R C 1 7 MCQ XL R A 1 8 MCQ XL R A 1 9 MCQ XL R B 1 10 NAT XL R 28.00 to 31.00 1 11 MCQ XL R D 2 12 MCQ XL R D 2 13 MCQ XL R C 2 14 MCQ XL R B 2 15 MCQ XL R C 2 16 MCQ XL R D 2 17 MCQ XL R A 2 18 MCQ XL R B 2 19 NAT XL R 20.25 to 20.25 2 20 NAT XL R 11.00 to 12.00 2 1 MCQ XL S B 1 2 MCQ XL S A 1 3 MCQ XL S A 1 4 MCQ XL S D 1 Q.No. Type Section Key/Range Marks 5 MCQ XL S D 1 6 MCQ XL S C 1 7 MCQ XL S D 1 8 MCQ XL S B 1 9 MCQ XL S A 1 10 NAT XL S 1.38 to1.42 1 11 MCQ XL S C 2 12 MCQ XL S C 2 13 MCQ XL S A 2 14 MCQ XL S D 2 15 MCQ XL S B 2 16 MCQ XL S A 2 17 NAT XL S 2.60 to 2.80 2 18 NAT XL S 0.5 to 0.5 2 19 NAT XL S 45.50 to 46.50 2 20 NAT XL S 30.5 to 31.5 2 1 MCQ XL T C 1 2 MCQ XL T B 1 3 MCQ XL T A 1 4 MCQ XL T D 1 5 MCQ XL T C 1 6 MCQ XL T D 1 7 MCQ XL T B 1 Q.No. Type Section Key/Range Marks 8 MCQ XL T A 1 9 MCQ XL T A 1 10 MCQ XL T C 1 11 MCQ XL T B 2 12 MCQ XL T D 2 13 MCQ XL T C 2 14 MCQ XL T C 2 15 MCQ XL T B 2 16 MCQ XL T D 2 17 MCQ XL T C 2 18 MCQ XL T A 2 19 NAT XL T 5270 to 5310 2 20 NAT XL T 0.056 to 0.062 2 1 MCQ XL U B 1 2 MCQ XL U A 1 3 MCQ XL U C 1 4 MCQ XL U A 1 5 MCQ XL U D 1 6 MCQ XL U D 1 7 NAT XL U 1.55 to 1.65 1 8 NAT XL U 103.0 to 103.2 1 9 NAT XL U 54 to 56 1 10 NAT XL U 0 to 0 1 Q.No. Type Section Key/Range Marks 11 MCQ XL U B 2 12 MCQ XL U C 2 13 MCQ XL U C 2 14 MCQ XL U A 2 15 MCQ XL U D 2 16 MCQ XL U A 2 17 MCQ XL U B 2 18 NAT XL U 9.8 to 10.2 2 19 NAT XL U 1.1 to 1.8 2 20 NAT XL U 10 to 10 2

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