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GATE 2018 : Life Sciences [Section S / Microbiology] (with Final Answer Keys)

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GATE 2018 XL (S): Q.1 Microbiology (XL-S) Q. 1 Q. 10 carry one mark each & Q. 11 Q. 20 carry two marks each. David Baltimore s classification of viruses is based on differences in (A) host cell receptors used by viruses (B) the pathways required to synthesize virus mRNA (C) the modes of transmission of viruses (D) the envelope proteins on the surface of viruses Q.2 Which of the following immune system components can function as an opsonin? (A) Antibodies (C) Histamines Q.3 (B) T-cell receptors (D) Interferons The oral polio vaccine (OPV) consists of (A) live attenuated virus (C) viral toxin Q.4 Which of the following eukaryotic cellular components carries out intracellular degradation during autophagy? (A) Nucleus Q.5 (B) protozoa (B) fungi (C) algae (D) bacteria (C) viruses (D) protozoa (B) X-rays (D) UV rays The primary product of nitrogen fixation is (A) N2 Q.9 (D) Lysosomes Which of the following is NOT an accepted method for sterilization? (A) Autoclaving (C) Gamma rays Q.8 (C) Ribosomes Binomial nomenclature has NOT yet been adopted for (A) bacteria Q.7 (B) Golgi bodies Analysis of DNA sequences suggest that eukaryotic mitochondrial genomes primarily originated from (A) fungi Q.6 (B) killed virus (D) viral capsid subunit (B) NH4+ (C) NO2 (D) NO3 In humans, the key stages in the life cycle of malarial parasites occur in (A) red blood cells and the liver (B) red blood cells and platelets (C) red blood cells and the pancreas (D) red blood cells and the gut XL-S 1/3 GATE 2018 Microbiology (XL-S) Q.10 You have a 50 mg/mL stock solution of arginine. To prepare 1 liter of growth medium for an arginine auxotroph that requires 70 g/mL of arginine, the volume of this stock solution that should be added is _____________ mL (up to 1 decimal point) . Q.11 Accumulating evidence suggest that Domain Archaea is more closely related to Domain Eukarya than to Domain Bacteria. Which of the following properties are shared between eukaryotes and archaea ? (i) Protein biogenesis (ii) Presence of sterol containing membranes (iii) Ribosomal subunit structures (iv) Adaptation to extreme environmental conditions (v) Fatty acids with ester linkages in the cell membrane (A) (ii), (iii) and (v) (C) (i) and (iii) (B) (i), (ii), (iv), and (v) (D) (iii) and (iv) Q.12 Match the antimicrobial agents in group I with their category/mode of action in group II. Group I Group II (i) Fluoroquinolones (p) beta lactam antimicrobial (ii) Amphotericin B (q) inhibition of protein synthesis (iii) Tetracycline (r) inhibition of nucleic acid synthesis (iv) Amoxicillin (s) antifungal agent (A) (i)-(q), (ii)-(s), (iii)-(r), (iv)-(p) (C) (i)-(r), (ii)-(s), (iii)-(q), (iv)-(p) (B) (i)-(s), (ii)-(r), (iii)-(p), (iv)-(q) (D) (i)-(s), (ii)-(r), (iii)-(q), (iv)-(p) Q.13 Match the microorganisms to their predominant modes of transmission. Microorganism Mode of Transmission (i) Bordetella pertussis (p) Vector-borne (ii) Dengue virus (q) Blood-borne (iii) Entamoeba histolytica (r) Droplet infection (iv) Hepatitis B virus (s) Contaminated food (A) (i)-(r), (ii)-(p), (iii)-(s), (iv)-(q) (C) (i)-(q), (ii)-(p), (iii)-(s), (iv)-(r) (B) (i)-(s), (ii)-(q), (iii)-(p), (iv)-(r) (D) (i)-(s), (ii)-(r), (iii)-(p), (iv)-(q) Q.14 Match the precursors/intermediates with the corresponding metabolic pathways. Precursor/Intermediates Metabolic pathway (i) Inosine monophosphate (p) L-methionine biosynthesis (ii) Ornithine (q) L-tryptophan biosynthesis (iii) Chorismate (r) Purine biosynthesis (iv) Homocysteine (s) L-arginine biosynthesis (A) (i)-(q), (ii)-(r), (iii)-(s), (iv)-(p) (C) (i)-(r), (ii)-(p), (iii)-(s), (iv)-(q) XL-S (B) (i)-(p), (ii)-(r), (iii)-(s), (iv)-(q) (D) (i)-(r), (ii)-(s), (iii)-(q), (iv)-(p) 2/3 GATE 2018 Microbiology (XL-S) Q.15 Match the scientists to their area of major contribution Scientists Area of major contribution (i) Antonie van Leeuwenhoek (p) Taxonomy (ii) Carl Linnaeus (q) Antimicrobial agents (iii) Sir Alexander Fleming (r) Vaccination (iv) Louis Pasteur (s) Microscopy (A) (i)-(s), (ii)-(q), (iii)-(p), (iv)-(r) (C) (i)-(p), (ii)-(s), (iii)-(r), (iv)-(q) (B) (i)-(s), (ii)-(p), (iii)-(q), (iv)-(r) (D) (i)-(q), (ii)-(p), (iii)-(r), (iv)-(s) Q.16 Which of the following combinations would improve the resolution of a microscope? (i) Increasing the half aperture angle of the objective lens (ii) Decreasing the wavelength of the illumination source (iii) Decreasing the numerical aperture of the objective lens (iv) Decreasing the refractive index of immersion medium (A) (i) and (ii) (B) (ii) and (iii) (C) (ii) and (iv) (D) (i) and (iii) Q.17 Active transport involves the movement of a biomolecule against a concentration gradient across the cell membrane using metabolic energy. If the extracellular concentration of a biomolecule is 0.005M and its intracellular concentration is 0.5M, the least amount of energy that the cell would need to spend to transport this biomolecule from the outside to the inside of the cell is ________ kcal/mol (up to 2 decimal points). (Temperature T = 298K and universal gas constant R= 1.98 cal/mol K) Q.18 A continuous cell culture being carried out in a stirred tank reactor is described in terms of its cell mass concentration X and substrate concentration S. The concentration of the substrate in the sterile feed stream is SF = 10 g/L and yield coefficient Yx/s = 0.5. The flow rates of the feed stream and the exit stream are equal (F=5 mL/min) and constant. If the .3 specific growth rate (h-1) = , the steady state + SF = 10 g/L X, S V=3L concentration of S is _____ g/L (up to 1 decimal point). Q.19 The initial concentration of cells (N0) growing unrestricted in a culture is 1.0 106 cells/mL. If the specific growth rate ( ) of the cells is 0.1 h-1, the time required for the cell concentration to become 1.0 108 cells/mL is _________ hours (up to 2 decimal points). Q.20 The following stoichiometric equation represents the conversion of glucose to lactic acid in a cell: Glucose + 2Pi + 2ADP 2Lactate + 2ATP +2H2O If the free energy of conversion of glucose to lactic acid only is G0 = 47000 cal/mol, the efficiency of energy transfer is _______ % (up to 1 decimal point). ( G0 for ATP hydrolysis is 7.3 kcal/mol) END OF THE QUESTION PAPER XL-S 3/3 Q.No. Type Section Key/Range Marks 1 MCQ GA A 1 2 MCQ GA C 1 3 MCQ GA B 1 4 MCQ GA B 1 5 MCQ GA B 1 6 MCQ GA A 2 7 MCQ GA D 2 8 MCQ GA D 2 9 MCQ GA B 2 10 MCQ GA C 2 1 MCQ XL P A 1 2 MCQ XL P D 1 3 MCQ XL P D 1 4 NAT XL P 11 to 11 1 5 NAT XL P 4 to 4 1 6 MCQ XL P D 2 7 MCQ XL P D 2 8 MCQ XL P A 2 9 MCQ XL P D 2 10 MCQ XL P A 2 11 MCQ XL P C 2 12 MCQ XL P B 2 13 NAT XL P 1.39 to 1.43 2 Q.No. Type Section Key/Range Marks 14 NAT XL P 7.39 to 7.54 2 15 NAT XL P 13.40 to 13.36 2 1 MCQ XL Q B 1 2 MCQ XL Q A 1 3 MCQ XL Q C 1 4 MCQ XL Q C 1 5 MCQ XL Q D 1 6 MCQ XL Q C 1 7 MCQ XL Q D 1 8 MCQ XL Q B 1 9 NAT XL Q 12 to 12 1 10 NAT XL Q 50 to 50 1 11 MCQ XL Q A 2 12 MCQ XL Q A 2 13 MCQ XL Q D 2 14 MCQ XL Q B 2 15 MCQ XL Q B 2 16 NAT XL Q 512 to 512 2 17 NAT XL Q 20 to 20 2 18 NAT XL Q 0.8 to 0.8 2 19 NAT XL Q 77 to 77 2 20 NAT XL Q 8862 to 8862 2 1 MCQ XL R A 1 Q.No. Type Section Key/Range Marks 2 MCQ XL R B 1 3 MCQ XL R C 1 4 MCQ XL R D 1 5 MCQ XL R B 1 6 MCQ XL R C 1 7 MCQ XL R A 1 8 MCQ XL R A 1 9 MCQ XL R B 1 10 NAT XL R 28.00 to 31.00 1 11 MCQ XL R D 2 12 MCQ XL R D 2 13 MCQ XL R C 2 14 MCQ XL R B 2 15 MCQ XL R C 2 16 MCQ XL R D 2 17 MCQ XL R A 2 18 MCQ XL R B 2 19 NAT XL R 20.25 to 20.25 2 20 NAT XL R 11.00 to 12.00 2 1 MCQ XL S B 1 2 MCQ XL S A 1 3 MCQ XL S A 1 4 MCQ XL S D 1 Q.No. Type Section Key/Range Marks 5 MCQ XL S D 1 6 MCQ XL S C 1 7 MCQ XL S D 1 8 MCQ XL S B 1 9 MCQ XL S A 1 10 NAT XL S 1.38 to1.42 1 11 MCQ XL S C 2 12 MCQ XL S C 2 13 MCQ XL S A 2 14 MCQ XL S D 2 15 MCQ XL S B 2 16 MCQ XL S A 2 17 NAT XL S 2.60 to 2.80 2 18 NAT XL S 0.5 to 0.5 2 19 NAT XL S 45.50 to 46.50 2 20 NAT XL S 30.5 to 31.5 2 1 MCQ XL T C 1 2 MCQ XL T B 1 3 MCQ XL T A 1 4 MCQ XL T D 1 5 MCQ XL T C 1 6 MCQ XL T D 1 7 MCQ XL T B 1 Q.No. Type Section Key/Range Marks 8 MCQ XL T A 1 9 MCQ XL T A 1 10 MCQ XL T C 1 11 MCQ XL T B 2 12 MCQ XL T D 2 13 MCQ XL T C 2 14 MCQ XL T C 2 15 MCQ XL T B 2 16 MCQ XL T D 2 17 MCQ XL T C 2 18 MCQ XL T A 2 19 NAT XL T 5270 to 5310 2 20 NAT XL T 0.056 to 0.062 2 1 MCQ XL U B 1 2 MCQ XL U A 1 3 MCQ XL U C 1 4 MCQ XL U A 1 5 MCQ XL U D 1 6 MCQ XL U D 1 7 NAT XL U 1.55 to 1.65 1 8 NAT XL U 103.0 to 103.2 1 9 NAT XL U 54 to 56 1 10 NAT XL U 0 to 0 1 Q.No. Type Section Key/Range Marks 11 MCQ XL U B 2 12 MCQ XL U C 2 13 MCQ XL U C 2 14 MCQ XL U A 2 15 MCQ XL U D 2 16 MCQ XL U A 2 17 MCQ XL U B 2 18 NAT XL U 9.8 to 10.2 2 19 NAT XL U 1.1 to 1.8 2 20 NAT XL U 10 to 10 2

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