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2003 Course Analytical Biotechnology

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Total No. of Questions : 12] [Total No. of Pages : 2 [3864] - 439 P 1283 B.E. (Biotechnology) ANALYTICAL BIOTECHNOLOGY (2003 Course) (Sem. - I) (416287) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Answer three questions from Section I and three questions from Section II. 2) Answer to the two sections should be written in separate answer books. 3) Neat diagrams should be drawn whenever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) What is PCR? Discuss in detail the technique and its optimization. [18] OR Q2) Write notes on a) Snager s DNA sequencing. b) Southern blotting. [18] Q3) Describe in detail following DNA modifying enzymes a) DNA ligase. b) DNA polymerase. [16] OR Q4) State the experiment leading to invention of restriction enzymes. How many types of restriction enzymes are known? Which of these have a role in cloning? Briefly describe the role of enzyme. [16] Q5) What are Plasmids and Cosmids? Describe both in terms of structure and function. [16] OR Q6) Explain the following a) BAC vectors. b) Expression Vectors. [16] P.T.O.

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