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2003 Course Novel Separation Techniques

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Total No. of Questions : 12] P1167 [Total No. of Pages : 3 [3864] - 434 Final Year B.E. (Biotechnology) NOVEL SEPARATION TECHNIQUES (416284) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Figures to the right indicate full marks. 2) Use of Programmable calculator is not allowed. 3) Draw a neat sketch wherever necessary. 4) Make necessary assumptions wherever required. 5) Answer any THREE questions from section I and any THREE questions from section II. SECTION - I Q1) a) Explain the range and characteristics of Bioproducts? [6] b) Explain in detail the need of downstream processing? [10] OR Q2) a) b) Q3) a) b) Give a brief overview of Bioseparations? [6] Differentiate between bioprocesses and other conventional separation processes? [10] Explain in detail Gas chromatography with a neat sketch? [8] Define Ion exchange chromatography? What is the principle behind it? List out the applications of Ion exchange chromatography. [8] OR Q4) a) What are the materials used in HPLC columns for stationary phase and mobile phase? [4] b) How many types of column chromatography techniques are there? Explain the principle for separation behind column chromatography? [6] c) Explain Liquid chromatography with a neat sketch? [6] P.T.O.

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