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2003 Course Bioinformatics & Regulations

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Total No. of Questions : 10] [Total No. of Pages : 2 [3864]-440 P1294 B.E. (Biotechnology) BIOINFORMATICS AND REGULATIONS (2003 Course) (416288) (Sem. - II) Time : 3 Hours] [Max. Marks :100 Instructions to the candidates: 1) Answer three questions from Section I and three questions from Section II. 2) Answer to the two sections should be written in separate answer books. 3) Neat diagrams should be drawn whenever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) Define databases. Classify nucleotide databases. Write in detail about Secondary nucleotide databases. [18] OR Q2) What is BLAST? Explain in detail its working. [18] Q3) Align the following two sequences using Needleman wunsch algorithm, and write the optimal alignment. [Match - 2, Mismatch - 1. Gap - 1] Seq. 1 GTTG Seq. 2 GTG [16] OR Q4) What is SQL? What are different formats used for representation of a sequence? [16] Q5) Write short notes. (Any two) 8 marks each. a) Global alignment. b) Object oriented database. c) Specialized genomic recourses. d) Secondary protein databases. [16] P.T.O. SECTION - II Q6) What is Dot plot analysis? Explain with suitable example. [18] OR Q7) Explain in detail various regulatory requirements involved in starting of a biotechnology company. [18] Q8) Write in detail profiles of various companies involved in bioinformatics software development. [16] OR Q9) Explain in detail various methods of phylogenetic analysis. [16] Q10)Write short notes. (Any Two) 8 marks each a) Phase II trials. b) cGMP. c) Distance method. d) Epitope prediction. E [3864]-440 E 2 E [16]

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