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2003 Course Bioinformatics

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Total No. of Questions : 12] P957 [Total No. of Pages : 3 [3664]-352 B.E. (Information Technology) BIOINFORMATICS (2003 Course) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates : 1) Answer three questions from section I and three questions from Section II. 2) Answers to the two sections should be written in separate books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. 5) Assume suitable data, if necessary. SECTION - I Q1) a) Define bioinformatics. Mention and explain its various applications.[9] b) Explain the major types of protein databases with most suitable example for each. [7] OR Q2) a) Explain how molecular biology is considered as an information science. Also explain central dogma of molecular biology with neat diagram.[8] b) What is genomics? Explain the difference between structural & functional genomics. State the tools & techniques included in both. [8] Q3) a) What is structure visualization? State & explain the various features of representative protein structure rendering programs and compare them.[9] b) Explain user interface and information theory. Also explain the four basic components in user interface hierarchy with neat diagram. [8] OR Q4) a) b) Describe the working of microarray with spotting technique. What are the sources of variability in spotting? Compare spotting and affimetrix microarray preparation process. [9] Explain various data mining methods with neat diagrams. [8] P.T.O. Q5) a) Explain centralized and distributed data mining infrastructure in detail.[8] b) What are the types of machine learning processes? Explain any three machine learning techniques in detail. [9] OR Q6) a) What is text mining? Explain the NLP process of text mining with its various phases, in detail. [8] b) List different computational methods of sequence alignment. Explain any two of them. [9] SECTION - II Q7) a) What are the different methods of protein structure prediction? Explain the Ab Initio method of protein structure prediction process with the help of neat diagrams. [7] b) What are the components involved in a modelling and simulation system? Explain the basic modelling and simulation process in regards to bioinformatics with neat diagram. [10] OR Q8) a) Write short notes on:i) Collaboration and communication model. [5] ii) Synchronous and asynchronous collaboration. [5] b) Explain the comparative modelling process of protein structure prediction. Discuss all its phases in detail. [7] Q9) a) Explain FASTA algorithm. What FASTA programs are available for [6] sequence alignment? b) What are the recommended steps for FASTA search? [6] c) Compare FASTA and BLAST tools for sequence alignment. [5] OR Q10)a) Explain BLAST algorithm in detail with neat diagrams. [6] b) What is an E ( ) value? Explain its significance giving suitable examples. [4] c) Explain gapped - BLAST, alongwith all the major refinements included. What is filtering in BLAST? [7] [3664] - 352 2 Q11)a) Explain the process of interchange and transformation of pollutants in atmosphere, hydrosphere and lithosphere. [8] b) Define Biotechnology. What is the significance of environmental biotechnology? Discuss various factors responsible for degradation of the ecosystem. [8] OR Q12)a) Write short notes on:i) [5] ii) b) Genetic Markers. Polymerase Chain Reaction. [5] Explain various applications of genetic engineering. Y [3664] - 352 3 [6]

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