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2003 Course Bio-Process Equipment Design

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Total No. of Questions : 12] [Total No. of Pages : 4 [3664]-363 P1172 B.E. (Biotechnology) BIO-PROCESS EQUIPMENT DESIGN (416283) (2003 Course) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates : 1) Figures to the right indicate full marks. 2) Use of programmable calculator is not allowed. 3) Draw a neat sketch wherever necessary. 4) Make necessary assumptions wherever required. 5) Answer any Three questions from Section I and any Three questions from Section II. SECTION - I Q1) a) Describe the design features of agitated Nutsche filter. [6] b) Explain the cake discharge arrangement used in Rotary drum filter. [8] c) Explain Recessed plate construction of plate and frame filters. [4] OR Q2) a) Define following terms: i) Partition coefficient. iii) Retention time. [8] ii) Retention Volume. iv) Relative retention. b) State the advantages of High performance thin layer chromatography.[4] c) A chromatographic separation of two component samples on a 40 cm column gave the retention times for two solutes as 4 and 6 min. respectively. Calculate the following: [6] i) No. of theoretical plates. ii) Plate height. iii) Resolution of peaks. The base width of two chromatographic peaks are 0.2 and 0.4 min. Q3) a) b) State the need of downstream processing in the biological processes. Explain sublimation drying/Lyophilization. [8] Describe the general design procedure for Process equipment. [8] P.T.O.

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