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2003 Course Enzyme & Fermentation Eng.

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Total No. of Questions : 10] P1278 [Total No. of Pages : 3 [3664]-362 B.E. (Biotechnology) (Semester - I) ENZYME AND FERMENTATION ENGINEERING (2003 Course) (416282) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) 2) 3) 4) Answer three questions from Section I and three questions from Section II. Answers to the two sections should be written in separate answer books. Neat diagrams must be drawn wherever necessary. Figures to the right indicate full marks. SECTION - I Q1) a) Describe in detail the two methods of determining the constants in the Michaelis-Menten equation for kinetics of enzyme acting on one substrate. State the advantages of both the methods. [10] b) State the expression, application and limitations of the Monod s growth kinetic model. [6] OR Q2) a) Explain the kinetic implications of endogenous and maintenance metabolism with respect to microbial growth kinetics. [8] b) What are the different types of reversible inhibitions encountered in enzyme [8] systems? Write the rate expressions for each type. Q3) a) Differentiate between the two types of fermentation. Also state the advantages and disadvantages of both types. [12] b) Describe in brief the different types of continuous fermentation systems. [6] OR Q4) a) Explain the batch mode of fermenter operation. Also enlist its advantages and disadvantages. [6] b) Write notes on the following (any two) : i) Fed-batch mode of fermenter operation. ii) Hollow fibre bioreactors. iii) Tray Bioreactors. [12] P.T.O.

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