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2003 Course Enzyme & Fermentation Eng.

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Total No. of Questions : 10] P1278 [Total No. of Pages : 3 [3664]-362 B.E. (Biotechnology) (Semester - I) ENZYME AND FERMENTATION ENGINEERING (2003 Course) (416282) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) 2) 3) 4) Answer three questions from Section I and three questions from Section II. Answers to the two sections should be written in separate answer books. Neat diagrams must be drawn wherever necessary. Figures to the right indicate full marks. SECTION - I Q1) a) Describe in detail the two methods of determining the constants in the Michaelis-Menten equation for kinetics of enzyme acting on one substrate. State the advantages of both the methods. [10] b) State the expression, application and limitations of the Monod s growth kinetic model. [6] OR Q2) a) Explain the kinetic implications of endogenous and maintenance metabolism with respect to microbial growth kinetics. [8] b) What are the different types of reversible inhibitions encountered in enzyme [8] systems? Write the rate expressions for each type. Q3) a) Differentiate between the two types of fermentation. Also state the advantages and disadvantages of both types. [12] b) Describe in brief the different types of continuous fermentation systems. [6] OR Q4) a) Explain the batch mode of fermenter operation. Also enlist its advantages and disadvantages. [6] b) Write notes on the following (any two) : i) Fed-batch mode of fermenter operation. ii) Hollow fibre bioreactors. iii) Tray Bioreactors. [12] P.T.O. Q5) a) How is the power consumption of an agitator affected in the presence of gas in a fermenter system? Give correlations for calculating power required in the presence and absence of gas. [8] b) What are the different factors which cause a change in rheology of a fermentation broth? [8] OR Q6) a) Describe in detail the dynamic method of gassing out for the determination [8] KLa. Also state its advantages and disadvantages. b) Explain the procedure for the measurement and control of pH during a fermentation operation. [8] SECTION - II Q7) Write notes on the following (any four) : [16] a) Thermal death kinetics. b) HTST sterilization. c) Filter sterilization of media. d) Process variables used for fermenter scale-up. e) Factors affecting scale-up of fermenter systems. f) Advantages and disadvantages of direct steam sterilization. Q8) a) Differentiate between the physical and chemical methods of enzyme immobilization. [8] b) Write notes on the following : i) Medical applications of immobilized enzymes. ii) Utilization and regeneration of co-factors. [10] OR Q9) a) What are the advantages offered by immobilized enzyme over soluble enzyme? Enlist few applications of immobilized enzymes. [6] b) What are the factors which affect diffusion of a substrate when an enzyme is immobilized on the internal surface of the support? [6] c) Derive an expression for kinetics of immobilized enzymes when the enzyme is bound to the external surface of the support. [6] [3664]-362 2 Q10) Write notes on the following (any four) : a) Disposable bioreactors. b) Advantages of Microfermentation. c) Design of bioreactors for animal cell cultures. d) Bioreactors for suspension culture of animal cells. e) Semi-synthetic fermentation process. f) Disadvantages of disposable reactors. [3664]-362 3 [16]

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