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2003 Course Novel Separation Techniques

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Total No. of Questions : 12] P1019 [Total No. of Pages : 3 [3664]-364 B.E. (Biotechnology) NOVEL SEPARATION TECHNIQUES (416284) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Figures to the right indicate full marks. 2) Use of programmable calculator is not allowed. 3) Draw a neat sketch wherever necessary. 4) Make necessary assumptions wherever required. 5) Answer any three questions from section-I and any three questions from section-II. SECTION - I Q1) a) Explain in detail the characteristics of fermentation broths? b) Explain the following terms : [10] [6] i) Classical Biotechnology. ii) Modern Biotechnology. iii) High value low volume products. OR Q2) a) Explain in detail Physical and Rheological characteristics of fermentation broths? List out the rheological laws applicable to fermentation broths? [10] b) Explain different stages involved in downstream processing in bioprocess industries? [6] Q3) a) What do you mean by HPLC? What are the applications of HPLC? [6] b) Explain in detail size exclusion chromatography with a neat sketch?[10] OR Q4) a) Explain the following terms : i) ii) iii) iv) v) [10] Liquid chromatography. Ion exchange resins. Gel filtration. Inorganic molecular sieves. Stationary supports in chromatography. P.T.O.

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