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2003 Course Novel Separation Techniques

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Total No. of Questions : 12] P1019 [Total No. of Pages : 3 [3664]-364 B.E. (Biotechnology) NOVEL SEPARATION TECHNIQUES (416284) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Figures to the right indicate full marks. 2) Use of programmable calculator is not allowed. 3) Draw a neat sketch wherever necessary. 4) Make necessary assumptions wherever required. 5) Answer any three questions from section-I and any three questions from section-II. SECTION - I Q1) a) Explain in detail the characteristics of fermentation broths? b) Explain the following terms : [10] [6] i) Classical Biotechnology. ii) Modern Biotechnology. iii) High value low volume products. OR Q2) a) Explain in detail Physical and Rheological characteristics of fermentation broths? List out the rheological laws applicable to fermentation broths? [10] b) Explain different stages involved in downstream processing in bioprocess industries? [6] Q3) a) What do you mean by HPLC? What are the applications of HPLC? [6] b) Explain in detail size exclusion chromatography with a neat sketch?[10] OR Q4) a) Explain the following terms : i) ii) iii) iv) v) [10] Liquid chromatography. Ion exchange resins. Gel filtration. Inorganic molecular sieves. Stationary supports in chromatography. P.T.O. b) Give a brief notes on instrumentation for HPLC with a neat sketch?[6] Q5) a) Explain in detail Piezodialysis? b) Explain the following : i) ii) [9] [9] Co coupled transport. Counter coupled transport. OR Q6) a) What are Membrane Contactors? Explain how the enzyme catalysts are incorporated in various types of membrane contactors? [10] b) Define a membrane? c) What are the criteria of membrane separation processes? [2] [6] SECTION - II Q7) a) Define Spectroscopy? [2] b) List out the applications of spectroscopy in various analytical studies? [4] c) Explain NMR spectroscopy in detail with a neat sketch? [10] OR Q8) a) Define AAS? b) What are the principles of AAS? c) Explain the following : i) ii) iii) iv) v) [2] [4] [10] Chemical shift. Atomization of sample. Resonance lines. Boltzmann distribution equation. Sensitivity. Q9) a) Define Adsorption? What is the principle behind adsorption? List out the applications of adsorption? [6] b) Explain the following : i) ii) iii) iv) v) [10] Differential heat of adsorption. Integral heat of adsorption. Freundlich Equation. Languid isotherm. Gibbs adsorption isotherm. OR [3664]-364 2 Q10) a) How do you classify adsorption operations? [4] b) Explain in detail with a neat sketch : [12] i) ii) Temperature swing adsorption. Pressure swing adsorption. Q11) a) Explain in detail zone refining? b) Explain in detail supercritical fluid extraction? [8] [10] OR Q12) a) Define reactive distillation? Explain in detail the principle behind it? What are its applications? [8] b) Give a short notes on : i) ii) [10] Molecular Sieves. Hyphenated techniques. xxxx [3664]-364 3

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