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Pune University - SY BSc (Sem - II) VOC BIOTECH : Paper - I, April 2010

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Total No. of Questions : 4] P512 [Total No. of Pages : 2 [3717]-237 S.Y. B.Sc. (Vocational) BIOTECHNOLOGY Voc-Biotech. : 221 - Plant and Animal Tissue Culture (Sem. - II) (Paper - I) (2008 Pattern) Time : 2 Hours] [Max. Marks : 40 Instructions : 1) All questions are compulsory. 2) 3) Figures to the right indicate full marks. All questions carry equal marks. Q1) Answer each of the following in 1-2 lines. a) Define cell lineage. b) Give role of CO2 incubator in animal tissue culture. c) Phenol red is added in animal cell culture medium. Why? d) What is mechanical disaggregation? e) Give any two applications of continuous cell lines. f) Define the term 'Callus'. g) Name the osmoticum used in plant tissue culture. h) What is hardening of plantlets? i) Give role of IAA in plant tissue culture. j) [10] What is incubation? Q2) Write short notes on any two of the following. a) Autoclave. b) Serum free media. c) [10] Cell repository. P.T.O. 1 Q3) Attempt any two of the following. [10] a) Give characteristics of transformed cells. b) What is subculturing? Explain it's necessity in plant tissue culture. c) Describe the method of surface sterilization of explant. Q4) What is organ culture? Discuss different methods of organ culture. [10] OR Describe in detail initiation and establishment of protoplast culture. [3717]-237 -22

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Additional Info : S.Y. B.Sc. Biotechnology (Semister - I), VOC - Biotech - 221 : Plant and Animal Tissue Culture (Paper - I) (New Course) (2008 Patern) (Vocational), Pune University
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