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Pune University - SY BSc (Sem - II) VOC INDUSTRIAL MICROBI : Paper - I, April 2010

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Total No. of Questions : 4] P515 [Total No. of Pages : 2 [3717]-242 S.Y. B.Sc. (Vocational) INDUSTRIAL MICROBIOLOGY VOC-IND-MIC-221 : Microbial Fermentations and Downstream Processing (Sem. - II) (2008 Pattern) (Theory Paper - I) Time : 2 Hours] [Max. Marks : 40 Instructions : 1) All questions are compulsory. 2) Figures to the right indicate full marks. 3) All questions carry equal marks. 4) Draw neat labelled diagrams wherever necessary. 5) Use of scientific calculators is allowed. Q1) Answer each sub-question in one or two lines; Fill in the blanks; State whether the statement is true or false. [10] a) Give another name for Vitamin B12. b) Name the product(s) produced by the action of beta-amylase on starch. c) Fill in the blank: enzyme can be used to lyse fungal cells. d) Fill in the blank: Ultrasonication cannot be used for lysing microbial cells in large scale operations because . e) State whether the statement is True or False. Glutamic acid is a primary metabolite. f) State whether the statement is True or False. At acidic pH, Penicillin gets converted to penicilloic acid. g) Define : 'Partition Coefficient'. h) Name the organism used as bioinoculant for solubilizing phosphate. P.T.O. 1 i) State the role of Kieselguhr in downstream processing of fermentation products. j) Name any two processes involved in the formulation of finished fermentation products. Q2) Answer any two of the following. [10] a) With the help of a diagram, explain the principle of filtration as a method of solids separation. b) Enlist the salts used for the precipitation of proteins from fermented broth. Describe the method of enzyme precipitation using salts. c) Explain principle of affinity chromatography with the help of suitable example. Q3) Answer any two of the following. [10] a) Draw the flow-chart for explaining steps involved in cheese production. b) What are bioinoculants? What are the advantages of using bioinoculants over their chemical counterparts? c) Explain the biochemistry involved in acetic acid production. Q4) Answer any one of the following. [10] a) With the help of a flow-sheet, describe the production of Penicillin G by fermentation. b) Describe the principle and working of centrifugation process used in down-stream processing. [3717]-242 -22

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Additional Info : S.Y. B.Sc. Industrial Microbiology (Semister - II), VOC - IND - MIC - 221 : Microbial Fermentations and Downstream Processing (Paper - I) (New Course 2008) (Vocational), Pune University
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