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Pune University - SY BSc (Sem - I) VOC INDUSTRIAL MICROBIO : Paper-II, April 2010

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Total No. of Questions : 4] P303 [Total No. of Pages : 2 [3717] - 149 S.Y. B.Sc. (Vocational) INDUSTRIAL MICROBIOLOGY VOC-IND-MIC -212: Screening & Process Optimization (Paper - II) (Semester - I) (New 2008 Course) Time : 2 Hours] [Max. Marks :40 Instructions: 1) All questions are compulsory. 2) Figures to the right indicate full marks. 3) All questions carry equal marks. 4) Draw neat labelled diagrams wherever necessary. 5) Use of scientific calculators is allowed. Q1) Answer each sub-question in one or two lines; Fill in the blanks; State whether the statement is true or false. [10] a) State whether the following statement is TRUE or FALSE. In Penicillin production, Corn Steep Liquor plays an important role. b) State whether the following statement is TRUE or FALSE. Peptones in nutrient media serve a dual purpose. c) Fill in the blank. _________ is used as a source of carbon as well as antifoam agent in fermentation media. d) Fill in the blank. _________ method is widely used for preservation of sporulating fungi. e) Fill in the blank. The _________ plate technique is used for isolation of analogue resistant mutants. f) Define Lyophilization . g) Define Del Factor . h) Define targeted screening. i) What does the term exhaust gas mean in fermentation? j) What is the role of dummy variables in Plackett-Burman Design for medium optimization. [ P.T.O. Q2) Write short notes on any two of the following. a) Secondary screening. b) Raw materials used as source of nitrogen in fermentation media. c) [10] Preservation of microbial cultures at very low temperature. Q3) Answer any two of the following [10] a) Explain how the dissolved oxygen concentration is measured and controlled during fermentation. b) Describe major factors involved in Scale-up. c) Explain the method to monitor and control foaming during fermentation. Q4) Answer any one of the following. [10] a) What are unculturable bacteria ? Describe newer cultivation approaches for these types of bacteria. b) What are auxotrophic mutants? Explain the use of the same for overproduction of primary metabolites. zzzz [3717]-149 -2-

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Additional Info : S.Y. B.Sc. Industrial Microbiology (Semister - I), VOC - IND - MIC - 212 : Screening & Process Optimization (Paper - II) (New Course 2008) (Vocational), Pune University
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