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Pune University - SY BSc (Sem - I) VOC INDUSTRIAL MICROBIO : Paper - I, April 2010

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Total No. of Questions : 4] P297 [Total No. of Pages :2 [3717] - 142 S.Y. B.Sc. (Vocational) INDUSTRIAL MICROBIOLOGY VOC - IND - MIC - 211 : Bioreactors - Design and Operation (Paper - I) (New Course 2008) (Sem. - I) Time : 2 Hours] Instructions to the candidates: 1) All questions are compulsory. 2) Figures to the right indicate full marks. 3) All questions carry equal marks. 4) Draw neat labeled diagrams wherever necessary. 5) Use of scientific calculators is allowed. [Max. Marks : 40 Q1) Answer each sub-question in one or two lines; Fill in the blanks; State whether the statement is true or false. [10] a) b) c) d) e) State whether the following statement is TRUE or FALSE Positive pressure is maintained within the head space during a fermentation to prevent contamination . State whether the following statement is TRUE or FALSE Foam formation during a fermentation is due to excess carbohydrate in the fermentation broth . State whether the following statement is TRUE or FALSE Baffles are used to smoothen the flow of broth within the fermenter . State whether the following statement is TRUE or FALSE Exit gas from a fermentation broth is a mixture of gases . State the disadvantage of batch process as compared continuous process of fermentation. f) Justify that Hollow Fibre Reactors are not preferred for high viscosity fermentation broths. g) Justify the following statement Heating / cooling jackets not used in production scale fermenters . h) List the type of fermenter in which non-mechanical agitation is practical. i) Define the term X90 value for fibrous filters. j) Define dual fermentation . P.T.O. Q2) Attempt any two of the following : [10] a) Draw a neat labeled diagram of a turbidostat and briefly explain how it differs from a chemostat. b) With the help of a diagram, explain the principle of operation of a sensor used for monitoring DO during a fermentation process. State the importance of monitoring DO. c) With the help of a diagram, explain the difference between a turbine impeller and a propeller impeller. Q3) Answer any two of the following : [10] a) Diagrammatically represent the four methods used for immobilization of cells and enzymes. Explain gel entrapment method of cell immobilization and its application(s) in the fermentation industry. b) With the help of a diagram, explain the construction and functioning of cooling towers. c) Explain the advantages of a continuous process of fermentation over a batch process. Draw the profile for product formation during a continuous process. Q4) Answer any one of the following : [10] a) Explain why monitoring of a fermentation is important. Draw a profile for the Time-Course of a batch process for production of biomass and delineate the important parameters that need to be monitored. b) Using a diagram, draw a fermenter outer vessel, impellers and baffles for a CSTR with a working volume of 10,000 litres. kbkb [3717]-142 2

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Additional Info : S.Y. B.Sc. Industrial Microbiology (Semister - I), VOC - IND - MIC - 211 : Bioreactors - Design and Operation (Paper - I) (New Course 2008) (Vocational), Pune University
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