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Pune University - SY BSc (Sem - I) VOC BIOTECH : Paper - II, April 2010

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Total No. of Questions : 4] [Total No. of Pages : 2 P299 [3717] - 144 S.Y. B.Sc. BIOTECHNOLOGY VOC. Biotech - 212 : RDT and Bioinformatics (Paper - II) (Sem. - I) (New Course) (Vocational) Time : 2 Hours] [Max. Marks : 40 Instructions to the candidates: 1) All questions are compulsory. 2) Figures to the right indicate full marks. 3) Neat diagrams must be drawn wherever necessary. 4) All questions carry equal marks. Q1) Answer each of the following in (1-2) lines : a) Give any two applications of RDT. b) What are restriction enzymes? c) Define cosmids. d) What is transfection? e) Enlist the components involved in PCR technique. f) What is site-directed mutagenesis? g) Define cloning vector. h) What is proteomics? i) Enlist any two phage vectors used in RDT. j) [10] What do you mean by Western blotting? Q2) Write short notes on Any Two of the following (8-10 lines) : a) YAC vectors. b) DNA modifying enzymes. c) [10] Transformation. P.T.O.

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Additional Info : S.Y. B.Sc. Biotechnology (Semister - I), VOC - Biotech - 212 : RDT and Bioinformatics (Paper - II) (New Course) (Vocational), Pune University
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