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Pune University - Microbial Metabolism, April 2010 (Old Course)

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Total No. of Questions : 5] P999 [Total No. of Pages : 2 [3725] - 23 M.Sc. MICROBIOLOGY MB - 603 : Microbial Metabolism (2005 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labeled diagrams wherever necessary. 4) Use of scientific calculator and log table is allowed. 5) Assume suitable data if necessary. Q1) Attempt any two of the following: [16] a) Describe the organization of cell membrane. b) What are the methods employed for the elucidation of a metabolic pathway? c) How is ammonia assimilated in the cell? Q2) Attempt any two of the following : a) State the significance of Gibbs free energy equation. b) Derive Hill equation. Draw Hill plot. State its significance. c) Compare bacterial and plant photosynthesis. [16] Q3) Attempt any two of the following : [16] a) Explain King & Altman method using single substrate enzyme catalyzed reaction. b) Describe the inhibitors of mitochondrial electron transport chain. c) Discuss the mechanism of energy generation in methanogenic bacteria. P.T.O. Q4) Write short notes on any four of the following : a) Laws of thermodynamics and their significance. b) Model membranes. c) Photolysis of water. d) Glutamate dehydrogenase. e) Nitrogenase. [16] Q5) Solve the following : [16] a) Calculate G of hydrolysis of ATP at pH 7.00 and at 25 C under steady state conditions in which concentration of ATP, DP and Pi, is maintained at 10-3 M, 10-4 M and 10-2 M respectively. (Given : G0 of ATP = 7.7 Kcal/M; R = 1.98cals/M). b) Predict what products will be rapidly labeled with 18O when following additions are made to actively photosynthesizing system. H2 18O is added to green plants. C18O2 is added to green plants. C18O2 is added to green sulfur bacteria. vvvv [3725]-23 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 603 : Microbial Metabolism (2005 Pattern), Pune University
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