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Pune University - Virology, April 2010 (Old Course)

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Total No. of Questions : 5] P997 [Total No. of Pages : 2 [3725] - 21 M.Sc. MICROBIOLOGY MB - 601 : Virology (2005 Pattern) Time : 3 Hours] Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Use of scientific calculators and log table is allowed. 4) Assume suitable data if necessary. 5) Draw neat labeled diagrams wherever necessary. [Max. Marks : 80 Q1) Attempt any two of the following: [16] a) What types of cell lines are used for cultivation of viruses? Give reasons for your answer. b) Discuss the one-step growth curve of bacteriophage. c) Explain how plant viruses can be detected using serological methods. Q2) Attempt any two of the following : [16] a) How animal viruses are classified? What are the ICTV recommendations for classification? b) Describe in detail, the life cycle of Rabies virus. c) Explain how vectors play an important role in spreading infection in plant viruses. Q3) Attempt any two of the following: [16] a) Describe the life cycle of TMV virus. b) Explain which combination of HA and NA is present in Swine flu influenza virus? Explain the role of HA and NA in the spread of influenza virus. c) Explain chemical assays used to detect the viruses. P.T.O. Q4) Write short notes on any four of the following : a) Indicator plants. b) Distinctive properties of viruses. c) LD 50. d) M13 phage. e) Interferon. [16] Q5) a) 107 cells of E.coli were exposed to T4 phage. At the end of the adsorption period there were 105 infected cells. What is the multiplicity of infection? [8] b) Determine the LD50 value from the following results of an experiment carried out on mice, where each dilution was tested on a set of 6 mice.[8] Virus dilutions No. of mice which died 10 -1 6 10 -2 5 10 -3 3 10 -4 1 10 -5 0 vvvv [3725]-21 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 601 : Virology (2005 Pattern), Pune University
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