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Pune University - Molecular Biology - I, April 2010 (Old Course)

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Total No. of Questions : 5] P1001 [Total No. of Pages : 2 [3725] - 32 M.Sc. MICROBIOLOGY MB - 702 : Molecular Biology - I (2005 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Neat well labeled diagrams must be drawn wherever necessary. 4) Use of log tables and electronic pocket calculators is allowed. 5) Assume suitable data, if necessary. Q1) Attempt any two of the following: [16] a) How is higher order structure of chromatin formed? b) Explain ATP dependent and ATP independent events in DNA replication. c) How mismatch repair of heteroduplex leads to gene conversion? Q2) Attempt any two of the following : a) How DNA replication is connected to cell cycle in E.coli? b) What are the characteristics of genetic code? c) [16] How Ras genes finely balanced at the edge of oncogenes? Q3) Comment on any two of the following : a) Error proof DNA repair mechanism. b) Tn 10 transposons. c) [16] C-value paradox. P.T.O. Q4) Write short notes on any four of the following : a) Sangers Di-deoxynucleotide method. b) Holliday Model of recombination. c) Base excision repair. d) Zinc motifs. e) P53 Proteins. [16] Q5) Dr. Franklin Stein, a classical anatomist by training, has developed an interest in genetic engineering and directed evolution. In a preliminary investigation of specific amino acid conversions, he has been studying a protein of unknown function from the bacteriophage . Using hydroxylamine, he isolates a mutant a, which makes only a fragment of the wild type protein. Upon treatment of mutant a, with 2-aminopurine, he is able to isolate two additional mutants, b and c, which also make only fragments of the wild type protein. Mutants a, b and c are nonviable on most of the bacterial strains, but can be propagated and distinguished by their growth on suitable nonsense-suppressing strains of bacteria as indicated in the following table. When mutant a is mated to either mutant b or c, no recombinants that will grow on an Su host are produced. However, viable recombinants are produced in a mating of mutants b and c. What one amino acid difference exists between the protein from the wild type bacteriophage and that from a variable recombinant from the mating of mutants b and c? Table : Growth of Three Bacteriophage Mutants on Non suppressing and Suppressing Bacterial Strains. [16] Su Su2 Su4 Su9 Mutant a - - + - Mutant b - + + - Mutant c - - - + Note : - : No growth; + : Growth vvvv [3725]-32 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 702 : Molecular Biology - I (2005 Pattern), Pune University
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