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Pune University - Microbial Technology, April 2010

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Total No. of Questions : 5] [Total No. of Pages : 3 P1017 [3725] - 403 M.Sc. MICROBIOLOGY MB - 803 : Microbial Technology (2008 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labeled diagrams wherever necessary. 4) Figures to the right indicate full marks. 5) Use of logarithmic tables, electronic pocket calculator is allowed. 6) Assume suitable data, if necessary. Q1) With the help of a diagram, describe the construction of an air-lift bioreactor. State the situations in which an air-lift bioreactor is used and explain the advantages of an air-lift bioreactor over a conventional CSTR. [16] OR Describe the production of Rifamycin. Delineate the critical operating parameters for Rifamycin production. Q2) Attempt any two of the following : [16] a) With help of a suitable example, explain the batch mode of operation of fermentation process. How is a batch mode more convenient as compared to fed-batch process? b) What is Validation in context with process qualification? Explain with a suitable example. c) Explain the principle, construction and operation of a DO sensor. P.T.O. Q3) Attempt any two of the following : [16] a) What is kLa? Explain its significance in determining aeration rate and how is it measured. b) Explain bioremediation with the help of a suitable example. c) Explain why the form of mycelial growth during a fermentation process is important in context with product yield. Q4) Write short notes on any four of the following : a) NRe. b) c) d) e) [16] Limitations of continuous culture in fermentation processes. OTR. Non-Newtonian fluids. Forms of IPR. Q5) The tables given below show the effect of additional carbon and nitrogen sources on chitinase production by Streptomyces sp. The basal medium used was Chitin-Yeast extract-Salts (CYS) medium. [16] Table 1 : Influence of additional carbon sources On chitinase production by Streptomyces [3725]-403 2 Table 2 : Influence of additional nitrogen sources On chitinase production by Streptomyces The salts added in the medium and their amounts (g/L) are as follows : 0.5; K2HPO4, 2.0; MgSO4. 7H2O, 1.0; and FeSO4.7H2O, 0.1 and final pH of the medium adjusted to 7.0. Interprete the results and answer the following question : 1. Give the composition of the ideal medium composition for production of chitinase? vvvv [3725]-403 3

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Additional Info : M.Sc. MICROBIOLOGY, MB - 803 : Microbial Technology (2008 Pattern), Pune University
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