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Pune University - Microbial Diversity and Taxonomy, April 2010

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Total No. of Questions : 5] [Total No. of Pages : 2 P1006 [3725] - 101 M.Sc. MICROBIOLOGY MB - 501 : Microbial Diversity and Taxonomy (2008 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labeled diagrams wherever necessary. 4) Use of logarithmic tables and scientific calculator is allowed. 5) Assume suitable data if necessary. Q1) Attempt any two of the following : [16] a) Describe the unique salient features of Archaea important in their taxonomy. b) Explain the importance of lipid analysis in bacterial taxonomy. c) Explain the various indices used to measure the microbial diversity. Q2) Attempt any two of the following : [16] a) Describe the advantages of RNA homology analysis over other conventional homology analysis in taxonomy. b) Explain using block diagram the full-length approach to characterize microorganisms without the need of cultivation. c) Describe the use of scoring matrices and gap penalties in sequence alignment. P.T.O. Q3) Attempt any two of the following : [16] a) Illustrate the major steps involved in rRNA sequencing to be applied for taxonomic studies. b) c) Explain the need and techniques of extracting total bacterial DNA from a habitat. Describe the proceedings involved in Needleman-Wunsch algorithm. Q4) Write short notes on any four of the following : a) Protein profiles in taxonomy. b) Chromosomal transfer as a tool in taxonomy. c) Application of FISH in bacterial diversity. d) Compare PSI-BLAST and PHI-BLAST. e) [16] Environmental clone libraries. Q5) A water sample from a sulfur spring was analyzed for its bacterial content. Microscopic observations indicated a bacterial load in the order of 106 cells/ ml. On examination by standard plating techniques on conventional nutrient media, the viable counts obtained were in the order of 104 CFU/ml. Explain the reason for the difference in count by these two methods. Describe the method(s) by which this difference in count could be nullified. [16] vvvv [3725]-101 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 501 : Microbial Diversity and Taxonomy (2008 Pattern), Pune University
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