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Pune University - Molecular Microbiology - II, April 2010 (Old Course)

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Total No. of Questions : 5] [Total No. of Pages : 2 P1005 [3725] - 43 M.Sc. MICROBIOLOGY MB - 803 : Molecular Biology - II (2005 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labelled diagrams wherever necessary. Q1) Answer any two of the following : [16] a) How does foot printing identify DNA binding sites for proteins? b) Give structural features of prokaryotic ribosome. c) Comment on : attenuation control in trp operon. Q2) Justify any two of the following : [16] a) Bacterial RNA polymerase is multimeric protein. b) Cosmid is a better vector than a plasmid. c) Pulse field gel electrophoresis is carried out for the DNA fragments with M.W. > 20kb. Q3) Schematically/diagrammatically represent any two of the following : [16] a) Dual control of arabinose operon. b) First two cycles in PCR. c) Cloning of human insulin gene in E.coli. P.T.O. Q4) Write short notes on any four of the following : a) Key features YAC. b) Fluorochromes in RT-PCR. c) Initiation factors in prokaryotic transcription. d) Molecular basis of antibody diversity. e) [16] Probes used in molecular biology. Q5) a) A gene encodes a polypeptide 30 amino acids long containing an alternating sequences of phe (UUU) and tyr (UAC) What are the sequences of nucleotides corresponding to this sequence in the following : i) The DNA strand that is read to produce the mRNA. ii) The DNA strand that is not read. iii) The anticodon on tRNA. b) [8] You have analyzed a cell lysate for its RNA components by various techniques. Following are the observations. Identify the type of molecule that best fits the observation giving reasons. Observation No. 1) A molecule with a short half life and heterogenous M.W. Observation No. 2) Molecules that form bands at distinct positions after centrifugation Observation No. 3) Molecules that form a diffused single band in gel electrophoresis. Observation No. 4) Molecules that form separate bands in gel electrophoresis. [8] vvvv [3725]-43 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 803 : Molecular Biology - II (2005 Pattern), Pune University
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