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Pune University - Microbial Metabolism, April 2010

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Total No. of Questions : 5] P1011 [Total No. of Pages : 2 [3725] - 203 M.Sc. MICROBIOLOGY MB - 603 : Microbial Metabolism (2008 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labeled diagrams wherever necessary. 4) Use of logarithmic tables and scientific calculator is allowed. 5) Assume suitable data, if necessary. Q1) Attempt any two of the following : [16] a) What is G, G and G '? What is their significance in biochemical reactions? b) Derive Adair equation for dimeric protein and state its significance. c) Describe the energy generation pathway in denitrifiers. Q2) Attempt any two of the following : [16] a) Justify : Aerobic microorganisms have more energy efficient metabolism than anaerobes. b) Justify : Concentration of reduced metabolites in the cell regulates photosynthetic activity in plants. c) How is ammonia assimilated into biomolecules? Q3) Attempt any two of the following : [16] a) Explain with the help of diagram nitrogen fixation in microorganisms. b) Derive the rate equation for competitive inhibition using King Altman s approach. c) Describe biosynthesis of aromatic amino acids. P.T.O. Q4) Write short notes on any four of the following : a) Water splitting complex. b) Oxidation reduction potential. c) Photosynthetic apparatus of bacteria. d) Ammonia oxidation. e) F1 - F0 ATPase. [16] Q5) Solve : [10] a) The following kinetic data were obtained for an enzyme in the absence of inhibitor (1) and in presence of inhibitor (2). Assume that [ET] is same for both experiments. Determine the Vmax and Km for the enzyme in presence and absence of inhibitor and comment on type of inhibition. [S] mol/L (2) V( mol/min) 1 10-4 28 17 1.5 10-4 36 23 2.0 10-4 43 29 5.0 10-4 65 50 7.5 10-4 b) (1) V( mol/min) 74 61 If for the half reactions the o' values are as follows [6] i) NAD+ + 2H+ + 2e- NADH + H+ o' = -0.32V. ii) -Ketoglutarate + CO2 + 2H+ + 2e- Isocitrate o' = -0.38V Calculate the G ' for the reaction catalyzed by isocitrate dehydrogenase in TCA cycle. (F = 96.485 KJ/V.mol, R = 8.314J/mol, Temp = 25 C). vvvv [3725]-203 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 603 : Microbial Metabolism (2008 Pattern), Pune University
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