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Pune University - Virology, April 2010

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Total No. of Questions : 5] P1014 [Total No. of Pages : 2 [3725] - 303 M.Sc. MICROBIOLOGY MB - 703 : Virology (2008 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Neat well labeled diagrams must be drawn wherever necessary. 4) Use of log tables and electronic pocket calculator is allowed. 5) Assume suitable data, if necessary. Q1) Attempt any two of the following : a) Explain in vivo technique for cultivation of viruses. b) Describe the pathophysiology of Herpes virus infection. c) [16] Comment on-Cellular sites for replication of plant viruses. Q2) Attempt any two of the following : [16] a) Explain the morphogenesis of T4 phage. b) How are nematodes responsible for transmission of plant viruses? c) What is micro-array DNA chip? Q3) Attempt any two of the following : [16] a) Comment on the need for development of recombinant DNA vaccine. b) How are indicator plants used to detect viruses? c) Name the criteria used for classifying animal viruses as per ICTV classification. P.T.O. Q4) Write short notes on any four of the following : a) Capsid symmetries. b) Primary cell lines. c) Epidemiology of Newcastle disease. d) Disease forecasting. e) EID 50. [16] Q5) A series of progressive dilutions of a viral stock was made in saline. 0.2ml of each dilution was injected in every mouse of a group of 10 mice. The mice were observed for infection. The observations are given in the following table. Find out the dose at which 50% of the mice shoed infection. [16] Dilution used Infection ratio 1:2 10/10 1:4 10/10 1:8 6/10 1:16 3/10 1:32 0/10 vvvv [3725]-303 2

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Additional Info : M.Sc. MICROBIOLOGY, MB - 703 : Virology (2008 Pattern), Pune University
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