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Pune University - Applied Microbial Biotechnology, April 2010 (Old Course)

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Total No. of Questions : 5] [Total No. of Pages : 3 P1003 [3725] - 41 M.Sc. MICROBIOLOGY MB - 801 : Applied Microbial Biotechnology (2005 Pattern) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) All questions are compulsory. 2) All questions carry equal marks. 3) Draw neat labeled diagrams wherever necessary. 4) Figures to the right indicate full marks. 5) Use of logarithmic tables, electronic pocket calculator is allowed. 6) Assume suitable data, if necessary. Q1) Draw a flow chart and describe the commercial production of Tetracycline. [16] OR Delineate the advantages using immobilized cells and enzymes for overproduction of microbial metabolites. With suitable examples, explain any two applications where immobilized enzymes are used. Q2) Attempt any two of the following : [16] a) Describe the design of and flow characteristics produced by a Marine propellor. b) Explain the concept of Reynolds Number and describe how it is significant in a fermentation process. c) Illustrate the concept of the 2-film theory of oxygen transfer to the cell from the bubble during aeration of a fermentation broth. P.T.O. Q3) Attempt any two of the following : [16] a) Draw a flow-chart for microbial leaching of copper. b) Explain the structure of the parasporal body of Bacillus thuringiensis and the action of the endotoxin. c) Draw and explain the construction of the DO probe. Q4) Write short notes on a ny four of the following : a) Siderophores. b) Use of Trichoderma in biocontrol. c) Role of PGPPs in metal sequestration. d) Power Number. e) [16] Advantages of synthetic vaccines. Q5) The Table and graphs shown below gives the production of Lipase in shake flasks, using different oils as substrates. [3725]-41 2 [16] Interprete the results and answer the following question : 1. Which would be the best substrate and medium composition (including initial pH) for maximum production of Lipase? Give your reasons. vvvv [3725]-41 3

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Additional Info : M.Sc. MICROBIOLOGY, MB - 801 : Applied Microbial Biotechnology (2005 Pattern), Pune University
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