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Pune University - FY BSc INDUSTRIAL MICROBIOLOGY - I, April 2010

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Total No. of Questions : 10] P210 [Total No. of Pages : 3 [3717] - 33 F.Y. B.Sc. INDUSTRIAL MICROBIOLOGY Instrumentation and Materials & Design (Theory Paper - I) (Vocational Course) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) Answers to the two sections should be written in separate books. 2) Neat diagrams must be drawn wherever necessary. 3) Figures to the right indicate full marks. 4) All questions carry equal marks. 5) Use of logarithmic tables, slide rule, Mollier charts, electronic pocket calculator and steam tables is allowed. 6) Assume suitable data, if necessary. 7) All questions are compulsory. SECTION - I Q1) Attempt the following : [8] a) Give composition of various supports used in thin layer chromatography. b) Define bed volume and void volume. c) Define Rf value. d) State Beers-Lambert s law. Q2) Attempt any two of the following : a) Explain the detection limits and sensitivity in instrumentation. b) Describe the technique of nephlometry c) [8] Describe the methods of pH and Eh measurements. P.T.O. Q3) Attempt any two of the following : a) Describe the working of conveyer discharge centrifuge. b) c) [8] Differentiate between paper chromatography and thin layer chromatography. Give the principle of polarimetry and describe the working in details. [8] Q4) Attempt any one of the following : a) Describe the method of purification and characterisation of a protein from a sample. b) Differentiate between preparative centrifuge and analytical centrifuge. Q5) Attempt any one of the following : a) Describe the role of isotopes in biological research. b) [8] Describe in details the technique of HPLC. SECTION - II Q6) Attempt the following : [8] a) b) c) What is metal alloy? Define Biofouling . Enlist various types of lives used in engineering drawing. d) What is Auto-CAD ? Q7) Attempt any two of the following : a) Describe die making for plastic molding. b) Explain heavy metal toxicity in aquatic environment. c) [8] Describe sectional lines & sectional views. [8] Q8) Attempt any two of the following : a) Describe the application of various ferrous and non ferrous metal alloys in biotechnological processess. b) c) What is difference between Pyramid and Prism. Describe the phenomenon of Oligo dynamic action . [3717]-33 2 Q9) Attempt any two of the following : [8] a) The major and minor axis of an ellipse measures 100mm and 70mm respectively. Draw an ellipse by rectangle method. b) Describe the plastic molding techniques used with respect to production of : i) Bottles ii) Pipettes. iii) Tubings. iv) Plates. c) Name different methods of drawing an ellipse. Q10)Attempt any one of the following : a) Describe advantages of Auto CAD over manual drawing methods. OR b) [8] Describe the microbial leaching of manganeese. vvvv [3717]-33 3

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Additional Info : F.Y. B.Sc. INDUSTRIAL MICROBIOLOGY Instrumentation and Materials & Design (Theory Paper - I) (Vocational Course), Pune University
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