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Pune University - FY BSc MICROBIOLOGY - II, April 2010

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Total No. of Questions : 5] P196 [Total No. of Pages : 2 [3717] - 18 F.Y. B.Sc. MICROBIOLOGY Basic Techniques in Microbiology (Paper - II) (New Course) (2008 Pattern) Time : 3 Hours] Instructions to the candidates: 1) All questions are compulsory. 2) Draw neat labelled diagrams wherever necessary. 3) Figures in bracket indicates full marks. [Max. Marks : 80 Q1) Attempt the following : [16] a) sin is the formula to calculate______ i) Resolving power. ii) Numerical aperture. iii) Angular aperture. iv) Total magnification. b) What is the role of Z.N.C.F. in spore staining. c) Give the formula for determining phenol coefficient of an unknown disinfectant. d) Name any two examples of photosynthetic bacteria. e) Give any two examples of Fluorescent dyes used in fluorescent microscopy. f) Glass lenses are used in electron microscope state whether true or false. g) Graphically show diauxic growth curve. h) What are accentuators. [16] Q2) Attempt any four of the following : a) Enlist different methods of preservation of pure culture. Describe any one in detail. b) Define selective media. Explain any one in detail. c) Draw and label various symbols to represent laboratory hazards. d) Describe the role of U.V. rays as sterilizing agent. e) What are mordants. Explain the role of mordants in staining procedure with example. f) Draw properly labelled ray diagram of dark field microscope. P.T.O. Q3) Attempt any four of the following : [16] a) Describe any one method of cultivation of chemolithotrophs. b) Describe the mode of action of halogen and halogen compounds as disinfectant. c) Define generation time. Derive the equation for its calculation. d) Explain the principle, methodology and application of negative staining. e) Justify : Blood agar is a enriched and differential media. f) Explain the various types of condensers used in bright field microscope. Q4) Attempt any two of the following : [16] a) Describe the nutritional classification of bacteria on the basis of carbon and Energy source. b) Enlist the different methods of enumeration of bacteria. Describe any one method in detail for counting cell number of bacteria. c) Enlist the different characteristics of ideal disinfectant. d) Define lense aberration. Describe chromatic aberration with diagramatic representation. Q5) Attempt any one of the following : [16] a) Explain principle, construction, working and applications of Transmission Electron Microscope. b) Define pure culture. Enlist different methods of getting pure culture. Describe any two in detail. vvvv [3717]-18 2

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Additional Info : F.Y. BSc. MICROBIOLOGY Basic Techniques in MICROBIOLOGY (Paper - I) (2008 Pattern)(New Course), Pune University
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