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Pune University - FY BSc MICROBIOLOGY - II (Old Course)

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Total No. of Questions : 5] P237 [Total No. of Pages : 2 [3717] - 69 F.Y. B.Sc. MICROBIOLOGY Basic Techniques in Microbiology (Paper - II) (Old Course) Time :3 Hours] Instructions to the candidates:1) All questions are compulsory. 2) Draw neat labelled diagrams wherever necessary. 3) Figures to the right indicate full marks. [Max. Marks :80 Q1) Attempt the following: [16] a) Define mordants with suitable example. b) maintains the isotonicity in nutrient agar. i) Peptone ii) Yeast extract iii) Sodium chloride iv) Water. c) Define zoonotic infection. d) Enlist the stains used for endospore staining by Schaffer & Fulton s method. e) Enlist any two gases used for disinfection. f) Define opportunistic pathogens. g) State true or false. i) Ionizing radiations have more penetration power than nonionizing radiations. ii) Use of moist heat is more beneficial than dry heat in sterilization process. h) enzyme is present in saliva. [16] Q2) Attempt any four of the following: a) Enlist various safety precautions to be taken in microbiological laboratory. b) Enlist various media used for cultivation of fungi. Describe any one in detail. c) Explain the role of rumen microflora in symbiotic association. d) With suitable example give nutritional classification of bacteria based on C & E source. e) Describe the role of various secretions in defense mechanism. f) Give the formula to calculate phenol coefficient. How will you comment on value of phenol coefficient? P.T.O Q3) Attempt any four of the following: [16] a) How will you cultivate alkalophiles in laboratory? Give any two examples of alkalophilic organisms. b) Describe the mode of action of chlorine as a disinfectant. c) Describe mycorrhiza as symbiotic association. d) Enlist various methods for isolation of bacteria. Describe any one in detail. e) Describe the principle of differential staining with suitable example. f) Define normal flora. Describe beneficial aspects of normal flora. Q4) Attempt any two of the following: [16] a) Give source, chemical nature & function of yeast extract & peptone. in bacteriological media. b) What are aberrations in objectives? Explain chromatic aberrations in detail. c) Explain the principle & observation for demonstration of capsule by Maneval s method. d) Describe the role of biological vectors in transmission of diseases. Q5) Attempt any one of the following: [16] a) Define disinfection. Explain the use of formaldehyde & halogens in disinfection procedure. b) With the help of ray diagram, explain the principle & working of dark field. Microscope. TTT [3717]-69 2

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Additional Info : F.Y. BSc. MICROBIOLOGY Basic Techniques in MICROBIOLOGY (Paper - II) (Old Course), Pune University
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