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Pune University - Tissue Culture (Plant and Animal), April 2010 (Old Course)

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Total No. of Questions : 8] P1279 [Total No. of Pages : 2 [3727-B]-71 M.Sc. BIOTECHNOLOGY BT-31 : Tissue Culture (Plant and Animal) (Old) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) Attempt a total of Five questions selecting at least two questions from each section. 2) Answers to the two sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) Enlist the types of tissue culture systems used in plant / animal tissue culture. Mention advantages, limitations and applications of any one system. [16] Q2) Mention the landmarks in the area of plant tissue culture. Explain any one recent development. [16] Q3) Explain the bases for selecting specific components for preparing plant tissue culture media. Add a note on significance of pH of nutrient medium. [16] Q4) Write explanatory notes on: a) Totipotency of plant cells. b) [16] Methods of separation of cell types. SECTION - II Q5) Compare and contrast in vitro organogenesis and embryogenesis in plants. [16] P.T.O. 1 Q6) Explain: a) b) [16] Secondary metabolism in cultured plant cells. Plant protoplast culture. Q7) Enlist the commercial applications of animal tissue culture and explain any one. [16] Q8) Write explanatory notes on: [16] a) Mammalian cloning. b) Genetic manipulation of cultured cells. [3727-B]-71 2 -2- Total No. of Questions : 6] P1280 [Total No. of Page : 1 [3727-B]-72 M.Sc. BIOTECHNOLOGY BT-32 : Fundamentals of Genetic Engineering (Old) Time : 1 Hours] [Max. Marks : 40 Instructions to the candidates: 1) Attempt a total of Four questions selecting at least two questions from each section. 2) Answers to the two sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) Explain the basic principles of engineering as applied to living system.[10] Q2) Enlist the methods of genetic engineering and explain any one. Cite suitable examples. [10] Q3) Write notes on: a) Screening of transformants. b) Site directed mutagenesis. [10] SECTION - II Q4) Enlist the expression vectors in bacteria. Explain organisation of any one vector. [10] Q5) Explain with suitable examples the mechanism of induced expression.[10] Q6) Write notes on: a) Chimeric constructs. b) Over expression of commercially important products. 1 [10]

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Additional Info : MSc BIOTECHNOLOGY, BT 31: Tissue Culture (Plant and Animal) (Old Course), Pune University
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