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Pune University - Tissue Culture (Plant and Animal), April 2010 (Old Course)

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Total No. of Questions : 8] P1279 [Total No. of Pages : 2 [3727-B]-71 M.Sc. BIOTECHNOLOGY BT-31 : Tissue Culture (Plant and Animal) (Old) Time : 3 Hours] [Max. Marks : 80 Instructions to the candidates: 1) Attempt a total of Five questions selecting at least two questions from each section. 2) Answers to the two sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) Enlist the types of tissue culture systems used in plant / animal tissue culture. Mention advantages, limitations and applications of any one system. [16] Q2) Mention the landmarks in the area of plant tissue culture. Explain any one recent development. [16] Q3) Explain the bases for selecting specific components for preparing plant tissue culture media. Add a note on significance of pH of nutrient medium. [16] Q4) Write explanatory notes on: a) Totipotency of plant cells. b) [16] Methods of separation of cell types. SECTION - II Q5) Compare and contrast in vitro organogenesis and embryogenesis in plants. [16] P.T.O. 1

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Additional Info : MSc BIOTECHNOLOGY, BT 31: Tissue Culture (Plant and Animal) (Old Course), Pune University
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