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Pune University - Sem - I : Advanced Biological Chemistry, April 2010

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Total No. of Questions :8] [Total No. of Pages : 2 P1196 [3727-B]-501 M.Sc. BIOTECHNOLOGY BT 11 : Advanced Biological Chemistry (2008 Pattern, New) (Sem. - I) Time :3Hours] [Max. Marks : 80 Instructions to the candidates: 1) Attempt a total of five questions selecting at least two questions from each section. 2) Answers to the two sections must be written on separate answer books. 3) Neat diagrams must be drawn wherever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) a) Explain NMR and IR spectroscopy have been proven to be valuable techniques in structural biology. [8] b) With the help of a schematic diagram, explain the components and working of spectro fluorometer. [8] Q2) a) Explain with the help of a suitable example, how pathway manipulation leads to qualitative and/or quantitative change in the final product. [8] b) Discuss the role of HPLC and GLC in analysis of a metabolite. [8] Q3) a) If the equilibrium concentration of ATP = 1.0 10 7 M, ADP = 0.165M and Pi = 0.1 M, what are the equilibrium constants and Go for the hydrolysis of ATP (25 C)? [8] b) What is meant by metabolic flux analysis? Mention, with suitable example, its significance. [8] Q4) a) Illustrate the principle of isopycnic density gradient centrifugation. State its applications. [8] b) Enlist the types of terpenoids mention four major steps involved in the biosynthesis of terpenoids. [8] P.T.O. SECTION - II Q5) a) Explain 2D gel electrophoresis as an appropriate tool to study proteins. [8] b) Compare and contrast integral proteins and peripheral proteins with respect to isolation, properties and functions. [8] Q6) a) Mention the phytochemical methods for analysis of natural products and explain any one. [8] b) Enlist the types of secondary metabolites and mention at least on pharmacological action of each. [8] Q7) a) b) Describe the role of chaperons in assisted protein folding. [8] Write an explanatory note on sequential model of allosterism. [8] Q8) a) Selecting an appropriate example, illustrate pathway manipulation at [8] cellular level. b) What are alkaloids? Mention their types. State pharmacological action of any four types of alkaloids. [8] #### [3727-B]-501 2

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Additional Info : MSc BIOTECHNOLOGY, BT 11 : Advanced Biological Chemistry (2008 Pattern, New) (Semister - I), Pune University
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