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GCE JUN 2008 : (AS 3) Practical Processes

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Centre Number 71 Candidate Number ADVANCED SUBSIDIARY (AS) General Certificate of Education 2008 Biology assessing Module 3A: Practical Processes ASB31 Assessment Unit AS 3A [ASB31] FRIDAY 13 JUNE, AFTERNOON TIME 1 hour. INSTRUCTIONS TO CANDIDATES Write your Centre Number and Candidate Number in the spaces provided at the top of this page. Write your answers in the spaces provided in this question paper. Answer all five questions. You are provided with Photograph 3.2 for use with Question 2 in this paper. Do not write your answers on this photograph. For Examiner s use only Question Number INFORMATION FOR CANDIDATES The total mark for this paper is 50. Quality of written communication will be assessed in questions 1(b), 3(b) and 5(c), (denoted in the paper with an asterisk, *) and awarded a maximum of 2 marks. Figures in brackets printed down the right-hand side of pages indicate the marks awarded to each question or part question. 1 2 3 4 5 QWC Total Marks ASB3AS8 3632 Marks 1 The diagram below shows some leaf pigments after separation by chromatography. Examiner Only Marks Remark solvent front carotene xanthophyll chlorophyll b origin line containing concentrated spot of leaf pigments (a) Describe how you would obtain a concentrated spot of leaf pigments on the chromatography paper. _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [2] * (b) Describe how you would set up and run the chromatogram to get a separation of the pigments as shown in the diagram above. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [3] ASB3AS8 3632 2 [Turn over (c) The spot for chlorophyll a has been omitted in the diagram opposite. With the solvent used, the Rf value for chlorophyll a was 0.65. Determine the distance moved by the spot, and indicate on the diagram the location of its mid-point. Show your working in the space below. Examiner Only Marks Remark [2] ASB3AS8 3632 3 [Turn over 2 Photograph 3.2 is a photomicrograph of part of a transverse section through the leaf of marram grass (Ammophila arenaria). Marram grass is common in sand dunes and has xerophytic adaptations. Examiner Only Marks Remark In the space below, draw a block diagram to show the tissue layers in the leaf as shown in the photograph. Annotate the drawing to identify two xerophytic features and, in each case, explain how the feature acts as an adaptation. [9] ASB3AS8 3632 4 [Turn over BLANK PAGE (Questions continue overleaf) ASB3AS8 3632 5 [Turn over 3 The diagram below shows an apparatus used to investigate the influence of external factors on the rate of photosynthetic oxygen release by the aquatic plant Elodea (Canadian pond weed). Examiner Only Marks Remark syringe tap calibrated capillary tube thermometer beaker gas bubbles (oxygen) boiling tube light source healthy strand of Elodea water containing dissolved NaHCO3 Source: A-level Biology, W.D. Phillips & T.J. Chilton, Oxford University Press, 1989 (a) Explain why the boiling tube containing the Elodea is placed in a beaker of water. _________________________________________________________ ______________________________________________________ [1] ASB3AS8 3632 6 [Turn over * (b) Describe how the apparatus is used to measure the rate of oxygen release by the aquatic plant. Examiner Only Marks Remark _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [4] (c) Explain why the rate of oxygen released does not equal the gross (true) rate of photosynthesis. _________________________________________________________ ______________________________________________________ [1] (d) In an investigation of the influence of light intensity and carbon dioxide on the rate of photosynthesis, describe how each of these factors may be varied. (i) Light intensity ______________________________________________________ ______________________________________________________ ______________________________________________________ ___________________________________________________ [2] (ii) Carbon dioxide ______________________________________________________ ______________________________________________________ ______________________________________________________ ___________________________________________________ [2] ASB3AS8 3632 7 [Turn over 4 Tribolium confusum is a small beetle which feeds on flour and can become a serious pest. An experiment was devised to determine the effect of varying the quantity of food available on subsequent population growth. Twenty pairs of beetles were used to inoculate one culture containing 200 g of flour and one of 50 g of flour, and the number of beetles counted over a period of 90 days. The results are shown in the table below. Time/days 0 15 30 45 60 75 90 Examiner Only Marks Remark Number of Tribolium beetles in 200 g of flour in 50 g of flour 40 40 38 32 210 68 770 139 864 281 838 312 873 295 (a) Plot the above results, using an appropriate graphical technique. (Use the graph paper opposite.) [7] (b) Identify two major trends that are evident in the results. 1. _______________________________________________________ __________________________________________________________ 2. _______________________________________________________ _______________________________________________________ [2] (c) Suggest an explanation for the differences in the growth of the Tribolium populations. _________________________________________________________ ______________________________________________________ [1] (d) Suggest how long it takes for adults to be produced from eggs. Explain your reasoning. _________________________________________________________ _________________________________________________________ ______________________________________________________ [2] ASB3AS8 3632 8 [Turn over ASB3AS8 3632 9 [Turn over 5 The distribution of plants in the herb layer of woodland is usually uneven, and is often linked to the availability of light. Preliminary work would suggest that wood sorrel (Oxalis acetosella) is more common in those parts of the open woodland that are shadiest. Examiner Only Marks Remark You are asked to devise a plan to investigate the effect of light intensity on the distribution of wood sorrel in open woodland . You are supplied with 1 1 quadrats ( m m) 2 2 a light meter (capable of measuring light intensity on an arbitrary scale, 0 to 100) (a) In this investigation identify the independent variable ______________________________________ dependent variable ____________________________________ [2] (b) Use your understanding of the factors (biotic and abiotic) affecting the distribution of plants to suggest why wood sorrel may more commonly be found in the shadiest of sites. _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [2] * (c) Briefly outline the plan of the investigation to be carried out. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [3] ASB3AS8 3632 10 [Turn over (d) Explain how you would present the results graphically, and suggest how the results could be interpreted. Examiner Only Marks Remark _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ ______________________________________________________ [3] Quality of written communication THIS IS THE END OF THE QUESTION PAPER ASB3AS8 3632 11 [Turn over S SB3AS8 531-006-1 A 3/07 3632 12 [Turn over GCE Biology Advanced Subsidiary (AS) Assessment Unit AS 3A Module 3A: Practical Processes Summer 2008 Photograph 3.2 Source: Sidney Moulds/Science Photo Library ASB3AS8 3632.02 ASB31IN1 (For use with Question 2)

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Additional Info : Gce Biology June 2008 Assessment Unit AS 3 Module 3A: Practical Processes
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