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GCE JUN 2007 : (AS 3) Practical Processes

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Centre Number 71 Candidate Number ADVANCED SUBSIDIARY (AS) General Certificate of Education 2007 Biology assessing Module 3A: Practical Processes ASB31 Assessment Unit AS 3A [ASB31] TUESDAY 19 JUNE, MORNING TIME 1 hour. INSTRUCTIONS TO CANDIDATES Write your Centre Number and Candidate Number in the spaces provided at the top of this page. Write your answers in the spaces provided in this question paper. Answer all five questions. You are provided with Photograph 3.2 for use with Question 2 in this paper. Do not write your answers on this photograph. For Examiner s use only Question Number 1 - 10.03/06EA 2 - 1.05/06GG INFORMATION FOR CANDIDATES The total mark for this paper is 50. Quality of written communication will be assessed in questions 1(c), 3(c) and 5(b) (denoted in the paper with an asterisk, *), and awarded a maximum of 2 marks. Figures in brackets printed down the right-hand side of pages indicate the marks awarded to each question or part question. 1 2 3 4 5 QWC Total Marks ASB3AS7 1571 Marks 1 The diagram below shows a simple respirometer containing woodlice. Examiner Only Marks screw clip Remark drop of coloured oil capillary tube scale clamp woodlice soda lime Source: Adapted from Advanced Biology Principles and Applications Study Guide, C. J. Clegg with D. G. Maclean, John Murray, 1996 (a) Describe how the woodlice affect the composition of the atmosphere within the apparatus. _________________________________________________________ _________________________________________________________ _______________________________________________________ [2] (b) Describe the role of soda lime in the apparatus. _________________________________________________________ _______________________________________________________ [1] * (c) Explain how the apparatus is used to measure the rate of respiration of the woodlice. _________________________________________________________ 1 - 10.03/06EA 2 - 1.05/06GG _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _______________________________________________________ [3] ASB3AS7 1571 2 [Turn over 2 Photograph 3.2 is a photomicrograph of a transverse section through the root of a dicotyledonous plant. The section includes a developing lateral root. Examiner Only Marks Remark 1 - 10.03/06EA 2 - 1.05/06GG In the space below, draw a block diagram to show the tissue layers in the root as shown in the photograph. Label the drawing to identify at least five structures. [9] ASB3AS7 1571 3 [Turn over 3 An experiment was undertaken to determine the water potential of potato tissue. Examiner Only Marks Remark The flow diagram below summarises the procedure used. Obtain samples of tissue of approximately equal size Cut tissue samples into slices Surface-dry slices using filter paper Weigh tissue sample slices Add to one of a series of sucrose solutions of known molarity Leave for 24 hours, after which surface-dry slices again and re-weigh Express the change in mass as a percentage of the initial mass (a) Explain each of the following steps in the procedure. how equal sized samples of tissue were obtained _______________________________________________________ _______________________________________________________ why the tissue sample was then cut into slices ______________________________________________________ _______________________________________________________ why the slices of tissue were surface-dried 1 - 10.03/06EA 2 - 1.05/06GG _______________________________________________________ _______________________________________________________ why the change in mass was expressed as a percentage _______________________________________________________ _____________________________________________________ [4] ASB3AS7 1571 4 [Turn over The results of the experiment are shown in the table below. Examiner Only Marks Remark Molarity of sucrose Initial mass/g Final mass/g % change in mass solution/mol dm 3 0 1.21 1.57 29.8 0.1 1.15 1.32 14.8 0.2 1.25 1.30 0.3 1.08 1.00 7.4 0.4 1.20 1.05 12.5 0.5 1.17 0.94 19.7 X= (b) Calculate the missing value, X, in the table. (Show your working in the space below.) [2] * (c) Describe how the results are used to determine the water potential of the potato tissue. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _______________________________________________________ [3] 1 - 10.03/06EA 2 - 1.05/06GG (d) In an evaluation of the experiment, the importance of a standardised drying technique was emphasised. Explain what is meant by a standardised drying technique and its importance to the accuracy of the result. _________________________________________________________ _________________________________________________________ _________________________________________________________ _______________________________________________________ [2] ASB3AS7 1571 5 [Turn over 4 The stomatal density within the upper and lower epidermis of the leaves of six plant species was investigated. The species involved may be categorised according to the orientation of their leaves, either upright or dorsi-ventral (horizontal): Upright leaves daffodil, maize, onion Dorsi-ventral leaves bean, oak, potato Examiner Only Marks Remark (a) Describe how the orientation of the leaves would affect the illumination of the leaf surfaces of the two groups of plants. _________________________________________________________ _______________________________________________________ [1] The results of the investigation are shown in the table below. Stomatal density/mm 2 Upper epidermis Lower epidermis Daffodil 76 36 Maize 50 70 Onion 176 176 Bean 40 280 Oak 4 360 50 160 Potato (b) Plot the above results, using an appropriate graphical technique. (Use the graph paper opposite). [7] (c) Identify the major trends evident in the results. _________________________________________________________ _________________________________________________________ _________________________________________________________ 1 - 10.03/06EA 2 - 1.05/06GG _______________________________________________________ [2] (d) Explain the role of stomata, and suggest an explanation for the distribution of stomata shown above. _________________________________________________________ _________________________________________________________ _________________________________________________________ _______________________________________________________ [2] ASB3AS7 1571 6 [Turn over ASB3AS7 1571 7 [Turn over 1 - 10.03/06EA 2 - 1.05/06GG 5 Cheese production relies upon the action of rennin enzymes which coagulate milk, turning it from a liquid into a solid. While a variety of rennins are available, two commonly used are fungal rennin (Fromase) and bovine rennin (from calves). The activity of the rennin can be determined by measuring how long it takes for the milk to solidify. Examiner Only Marks Remark You are asked to devise a plan to test the hypothesis that fungal rennin has a lower optimum temperature than bovine rennin. You are supplied with standard suspensions of fungal rennin and of bovine rennin milk general school laboratory apparatus (a) The fungus, Rhizomucor miehei, from which rennin (Fromase) is obtained, inhabits soil, while bovine rennin is obtained from the stomach of calves. Suggest an explanation for the formulation of the hypothesis stated above. _________________________________________________________ _________________________________________________________ _______________________________________________________ [1] * (b) Outline the procedure to be carried out. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ 1 - 10.03/06EA 2 - 1.05/06GG _________________________________________________________ _________________________________________________________ _______________________________________________________ [3] ASB3AS7 1571 8 [Turn over (c) Identify one major limitation in your procedure. Examiner Only Marks Remark _________________________________________________________ _________________________________________________________ _______________________________________________________ [1] (d) Complete the figure below to show how you would present your results graphically. [3] (e) Describe how the data obtained would be used to provide evidence for the hypothesis. _________________________________________________________ _________________________________________________________ _________________________________________________________ _______________________________________________________ [2] 1 - 10.03/06EA 2 - 1.05/06GG Quality of written communication THIS IS THE END OF THE QUESTION PAPER ASB3AS7 1571 9 [Turn over 1 - 10.03/06EA 2 - 1.05/06GG Permission to reproduce all copyright material has been applied for. In some cases, efforts to contact copyright holders may have been unsuccessful and CCEA will be happy to rectify any omissions of acknowledgement in future if notified. S 3/06 7-053-1 [Turn over GCE Biology Advanced Subsidiary (AS) Assessment Unit AS 3A Module 3A: Practical Processes Summer 2007 Photograph 3.2 1 11.3.06RTS (for use with Question 2) Source: Biophoto ASB3AS7 1571.02 ASB31IN1

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Additional Info : Gce Biology June 2007 Assessment Unit AS 3 Module 3A: Practical Processes
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