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GCE JUN 2007 : (AS 1) Cell Biology

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Centre Number 71 Candidate Number ADVANCED SUBSIDIARY (AS) General Certificate of Education 2007 Biology assessing Module 1: Cell Biology ASB11 Assessment Unit AS 1 [ASB11] MONDAY 4 JUNE, MORNING TIME 1 hour. INSTRUCTIONS TO CANDIDATES Write your Centre Number and Candidate Number in the spaces provided at the top of this page. Write your answers in the spaces provided in this question paper. Write your answer to Section B on the lined paper at the end of this booklet. Answer all eight questions. You are provided with Photograph 1.5 for use with Question 5 in this paper. Do not write your answers on this photograph. INFORMATION FOR CANDIDATES The total mark for this paper is 55. Section A carries 43 marks. Section B carries 12 marks. You should spend approximately 15 minutes on Section B. You are expected to answer Section B in continuous prose. Quality of written communication will be assessed in Section B. Figures in brackets printed down the right-hand side of pages indicate the marks awarded to each question or part question. ASB1S7 2340 For Examiner s use only Question Number 1 2 3 4 5 6 7 8 Total Marks Marks Section A Examiner Only Marks 1 Remark Identify the word or phrase that is described by each of the following statements. (a) A group of cells, all of which perform the same function. _______________________________________________________ [1] (b) A cellular process involving formation of a vesicle by the cell membrane, thereby enclosing the material to be taken into the cell. _______________________________________________________ [1] (c) The stage of mitosis during which the chromosomes line up at the cell equator. _______________________________________________________ [1] (d) An organelle, in both prokaryotic and eukaryotic cells, which is composed of protein and RNA. _______________________________________________________ [1] ASB1S7 2340 2 [Turn over 2 The diagram below represents the molecular structure of a triglyceride. Examiner Only Marks Remark HH HHH O O C C C H C O C C C C OH HH HHH H HHHHH O O H C O C C C C C C C HHHHH OH HHHHH O O H C O C C C C C C C H HHHHH OH (a) On the diagram, draw a line around an unsaturated fatty acid. [1] (b) Name the type of reaction used to attach a fatty acid to the glycerol molecule. _______________________________________________________ [1] (c) State one function of a triglyceride. _______________________________________________________ [1] (d) Describe how the structure and properties of a phospholipid differ from that of a triglyceride. __________________________________________________________ __________________________________________________________ __________________________________________________________ _______________________________________________________ [2] ASB1S7 2340 3 [Turn over 3 The diagram below shows part of a DNA molecule in the process of replication. Examiner Only Marks Remark 1 2 3 adenine guanine cytosine 4 (a) Name the structures labelled 1 to 4. 1 __________________________ 2 __________________________ 3 __________________________ 4 __________________________ [3] (b) Name the enzyme that catalyses the attachment of structure 4 to the DNA strand. _______________________________________________________ [1] ASB1S7 2340 4 [Turn over (c) What term is used to describe the mechanism by which DNA replicates? Examiner Only Marks Remark _______________________________________________________ [1] (d) When, precisely, during the cell cycle does DNA replication occur? _______________________________________________________ [1] ASB1S7 2340 5 [Turn over 4 Enzymes are globular proteins which catalyse metabolic reactions. The diagram below shows the arrangement of the amino acids in an enzyme to which substrates have attached. Examiner Only Marks Remark Y X (a) Name the part of the enzyme in which the amino acids are shaded grey. _______________________________________________________ [1] (b) Use the diagram and your understanding of enzyme action to explain how an enzyme acts as a catalyst. __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ _______________________________________________________ [3] (c) Identify the types of bond labelled X and Y. Bond X ___________________________________________________ Bond Y _________________________________________________ [2] ASB1S7 2340 6 [Turn over 5 Photograph 1.5 (magnification 30 000) represents part of two plant cells showing part of a chloroplast. Examiner Only Marks Remark (a) Identify the structures labelled A to D. A ___________________________ B ___________________________ C ___________________________ D ___________________________ [4] (b) The structure labelled C is 20 mm wide on the photograph. Use the magnification given to calculate the actual width of this structure in micrometres ( m). (Show your working in the space below.) Answer __________________ [2] ASB1S7 2340 7 [Turn over 6 The green plant, Nitella, absorbs ions from the pond water in which it lives. The concentration of three ions in pond water and in the cytoplasm of the plant cells is shown in the table below. Ion Concentration of ion in pond water /mmol dm 3 Concentration of ion in cell cytoplasm /mmol dm 3 3.0 10.8 3.6 Nitrate 0.05 2.5 50 0.008 Remark Accumulation ratio Magnesium Examiner Only Marks 1.8 Phosphate (a) Name the process by which the plant cells absorb these ions. What evidence is provided in the table to support your answer? __________________________________________________________ _________________________________________________________ __________________________________________________________ _______________________________________________________ [2] (b) The table also shows the accumulation ratio for two of the ions. Complete the table to show the missing value for phosphate. [1] (c) The ions are absorbed at different rates. Use your understanding of the functioning of the cell surface membrane to suggest an explanation for this. __________________________________________________________ _________________________________________________________ __________________________________________________________ _______________________________________________________ [2] ASB1S7 2340 8 [Turn over (d) The rate of absorption of all ions was found to differ in different plant cells. In fact, it was highest in cells with the greatest number of mitochondria. Suggest a reason for this. Examiner Only Marks Remark __________________________________________________________ _______________________________________________________ [1] (e) Give one function for the magnesium ions absorbed by the plant. __________________________________________________________ _______________________________________________________ [1] ASB1S7 2340 9 [Turn over 7 A 300 mmol dm 3 solution of salt (NaCl) has a solute (osmotic) potential of 1350 kPa. Examiner Only Marks Remark (a) State what could be added to this solution to raise the solute potential of the solution _____________________ to lower the solute potential of the solution __________________ [2] The spider crab (Maia) lives in the sea at a depth of over 30 metres, while the shore crab (Carcinus) lives in estuaries as well as along the shoreline. When the two species of crab are placed in dilute solutions of seawater the solute potential of their body fluids is altered. The results are shown in the graph below. 1000 1000 1400 Spider crab 1800 Shore crab 2200 Solute potential of body fluids/kPa undiluted seawater Solute potential of external solution/kPa 2600 2200 1800 1400 2600 (b) Using the graph, determine the solute potential of the body fluids of each species of crab when the seawater is diluted to a solute potential of 1600 kPa. ASB1S7 Spider crab __________________ Shore crab __________________ 2340 [2] 10 [Turn over (c) Describe and explain the effect of diluting seawater on the solute potential of the body fluids of the spider crab. Examiner Only Marks Remark __________________________________________________________ __________________________________________________________ __________________________________________________________ _______________________________________________________ [2] (d) When the shore crab is placed in dilute solutions of seawater it absorbs salt through its gills from the surrounding solution. (i) What is the effect of absorbing salt on the solute potential of the body fluids? ______________________________________________________ ____________________________________________________ [1] (ii) What is the evidence from the graph that the shore crab absorbs salt when placed in dilute solutions of seawater? ______________________________________________________ ____________________________________________________ [1] (e) Neither of these crabs survives when placed in very dilute seawater. What ultimately happens to animal cells when placed in solutions more dilute than the cell contents? __________________________________________________________ _______________________________________________________ [1] ASB1S7 2340 11 [Turn over Section B Examiner Only Marks Remark In this section you are expected to answer in continuous prose, supported, where appropriate, by diagrams. You are reminded that up to two marks in this question are awarded for the quality of written communication. [2] 8 Give an account of procedures used in recombinant DNA technology (genetic engineering) to include each of the following aspects. Isolation of a gene Use of a vector to incorporate the gene into a host cell [10] Isolation of a gene _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ ASB1S7 2340 12 [Turn over Examiner Only Marks Remark _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ Use of a vector to incorporate the gene into a host cell _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ _____________________________________________________________ ASB1S7 2340 13 [Turn over THIS IS THE END OF THE QUESTION PAPER ASB1S7 2340 14 [Turn over S 3/06 7-033-1 [Turn over GCE Biology Advanced Subsidiary (AS) Assessment Unit AS 1 Module 1: Cell Biology Summer 2007 Photograph 1.5 (For use with Question 5) B A D ASB1S7 2340 Source: George McCartney ASB11IN1 C

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Additional Info : Gce Biology June 2007 Assessment Unit AS 1 Module 1: Cell Biology
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