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CBSE Class XII 2011 : BIOTECHNOLOGY

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9 9 . No. o ~ -~ ~ C ~-~-a5T f5T n C ode t he ~ a nswer-book. w rite . C ode t he m u~t o f p age - a;)s t itle C andidates ~ ~an2ff t I he I I I I I I I I I I I I I I I N o. ~. ~. Roll '(f5)s I S S eries I OS - ~~fCir:i!1 attempting . b efore q uestion t he o f N umber S erial P lease . t Please check that this question paper contains 28 questions. he . d Code number given on the right hand side of the question paper should be written on the title page of the answer-book by the candidate. own . w Please check that this question paper contains 8 printed pages. rite . it. 15 minutes time has been allotted to read this question paper. The question paper will be distributed at 10.15 a.m. From 10.15 a.m. to 10.30 a.m., the students will read the question paper only and will not write any answer I ~ - q"{ ~ ~ -~ I ~ ~ o I ~ q;) 8 ~ 3" - ;p~ ~ , 2 8 m ~ ' # ~ ' # ~ ~ -~ ~ -~ ~ ~ ~ ~ f Cf) f Cf) ~ ~ ~ ~ ~ ~ ' ~ # ~ ~ ~ -~ ~-~~ ~ ~ o q;) ~ " qjT ~ -~ m ~ -~ I I ~ t " ~ (fq:j ~ ~ ~ ~ - --~ 1 \fJ.30 ~ ~ ~ " qjT - q"{ ~ f tRG 1 5 1 0.15 ~ BIOTECHNOLOGY -~"qjT I ~ ~ ~ ~ ~ ~ ~ ~ o q;) ~ ~ ~ 1 ~ 0.15 -~ ~ ~ this period. ~~~~~~~~~I~~~~~I ~ am '# . . . . . on the answer script during 99 1 7 0 7 0 . . 3 iq; M arks.. 3 M-;:r;r M aximum h ours 3 f !U"t 3 . . ~ a llowed.. Time ~-~i~lffl"a)') ~ r P.T.D. ~".","i""i~ ...: -c;if1r 99 - I - ; ~ 3 :nrr " ~ ~ 3 iCti m ;r - a fq; ~ ~ - i t; ~ - ~ q jT ~ J 3 1 - ? 9'175 i f m # I ~ ~ q nif a tq;7 3 ' Jft l og u se m ay questions, - i t; ' rft;r i t; ' im" y ou q uestions, a nswer s hort questions, fit ~ C fJ 4 ~ t i:fi'l: I ~ ; riff a lso a re 2 5 t o 1 6 n umber e ach. m arks 2 c arrying q uestions, a nswer s hort v ery a re 5 t o 1 n umber Q uestions D . a nd C B , A , - questions. Question paper contains four sections : i t ~ f qq;rr H owever, p ermitted. n ot i s e ach. m arks 3 Q uestions s uch i n c hoices t he o f o ne o nly a ttempt t o h ave Y ou marks. f ive o f q uestions t wo a nd m arks t hree o f q uesti,on o ne i n provided There is no overall choice. However, a~, internal t ~ i f ~ - ~ ~ ) ~ 3 #<7 I - ; e ach. carrying All questions are compulsory. (ii) ~ ~ f aRif t - ' ?JZR ' Ijtf:rff i f f iT ( 3i/qr f qq;rr c alculators m arks 5 (iii) (i) q i t i t f aRif I ~ ~ 3 -:rF: t t q jT G '7 q nif a tq;7 3 1Rqr2f o f U se answer 3 i t f aRif ~ ~ f qq;rr ~ - - ~ ~ \ ZrJtt answer 11 ~ f aRif t t < 1~rt<I{"i-{CfJ ~ i t; i t; n q;- 5 ,~rn<1~rt</r:l7CfJ J t C fJ { "i-{ 1 < r t ~ < 1 ' Jft ~ i t; n q;- 1 5 ~ 5 ~ q ;}f 26 to 28 are long ' ~ -drt<I\J;fCfJ ~ i t; n q;- 5 2 i t 1 ~ i f ~ 6 to 15 are short Jft t i:fi'l: I ~ C ff; rrr 3 Tl7ft7T " f:rq;i 2 8 i t i t 6 i !1iqc;r 31rrri!fiT number - q jT ) i t 6 1 m r ~ (v) Questions number q;-:( 3 Tl7ft7T 2 6 m r ~ Questions q ( ~ m r I ~' (vii) 1 mark jT 1 m r ~ tables, 4" t V < afq; 1 I ~' i (i) ) i (vi) t ~ (iii) (iv) i ~ (iv) ' ~'8?:ff (v) f ) (vi) ff /#-#/0<{ i (vi r General Instructions: choice has been each. carrying carrying if necessary. 'Rt;pr : I ~~~-;I afq;~1 -; I 2 -- t o r esistant c rops e ngineer t o u sed ~31' i s w hich t oxin b t acterium 1 "q)T ~ ~ ~ ~ ~~ ~ ~ 'srfuum ~ ~ b een h as w hich l aboratory t he i n b acterium a g row "qj) .3nl1 ~~I.I~II~I fCfim ~~I~~1-i g row t o i ncubator c ells r egular a a nimal i n t he p laced e xpect w ere y ou D 1 not? ~ '5rTUft ~~3rt ~~ ~ "qj) ~ ~ ~~ ~ ~~ ~ ~, ~~I~~I ~fuq)T3rt ~ ~ ~~"2""\ ~ \"@T ~ I~ ~ 3Tmr ~ ~ ~ - ~ '5rTUft ? ~~~m7ft~~ 4. o m edium c ells. c ulture a b acterial i n g c or rowing f or ells ' A used nimal 5fq)R~~? 1T1:; ~ ~ ("tifGf:5rT) ~ f ~ ~ s pring? h ot 1 ~ ~ a y ou f rom w ould ~~~~~I H ow isolated 2. 3. ~ "{fC(f In micropropagation apical meristems are used for raising virus-free 1 plants. Why? ~-~ ~ "qj)iiRR ~ ~ ' ~ ~ ~-~ ~3rt "q)T ~~ ~ ~ i rate (~) or doubling time (t), which one would be g rowth s pecific - t wo t he O f s lowly. g rows s pecies m icrobial g iven 5 ' A ~I"itm~? . f. he N ame 1. bollworms. ~ ~~I~~1-i ~ { r i A SECTION lower? 1 1 ~~~'GTfi- mlft ? "itm~? 99 t he i s W hat W hy? r -HuEPO. e xpress t o u sed i s l ine c 2 I ~ ~ ~ ~ " q)T ~ ~ m ~~ ' ~ 31~ 5rTUft protein? C of this HO r-HuEPO function ell a nimal C HO 6. ~'af SECTION ~ B ( ~~~ t), ~ ~ 3 r4qf ( ~) ~ ~ fqffip; ~~~~~~~~~mm~ f. ~~"q)Tq;r4~~? 3 P.T.D. 'q)T~'q:<:rl"~? ! 3 What I 1 l ower? g rowth s pecific - t wo t he O f ' ;j'fffir ~ ~ ~ ' q)T f q~art ~ i tmuft ~ ~3Trm~- ~ ~ ~ ~ q j)fu"q)Tart ~ ft"qr ~ , ~ g row t o c ells a nimal t he e xpect y ou D o c ells. b acterial g i ncubator r egular a i n p laced w ere m edium c ulture a ~ ~ ~ ~ IRq1{ f Cfim 7 fT; ~ ~ ~ ) ~ am ~I~I~TR"IT ~ ' I ~~~~ti- b e w ould ~ ~ are used for raising ;j'fffir Why? ? m 1fr R 1:;rn"{ ~ ~ o ne s lowly. ~ ~ ~ ~ . b een h as w hich l aboratory t he i n b acterium a g row u I ~ ~ ~ ~ "srfu'Um ~ ~ ~ ~ ~ ~ ~ "q)T toxin which is used to engineer ~ r-HuEPO. ~ to express ' B . q: , ) t ( ' GAR . I ~ . , ~~~~~G"{.q:OO~1 -.,:;I: I q)T ~'af w hich ~ meristems ~ ' qj)fuqjT m is used uft SECTION g rows n apical 0 H e I line ( ~ ~ - 1.;rT t), s pecies i al . ~ t ime l ing terium C ~ ~m~'i:ftJfr~mm~ 0 a hot ~ ~ a;riJ;qr u 1: ~3T A SECTION o ;,-'C - ~ ;;:;:i,~ '.~Iifi~i~ crops resistant to 1 spring? 1 ? 1 virus-free 1 is the s protein? 2 P. T.O. ~!" \ " i I I ; ,. I 2 c 3 q /r;{ q /r;{- i Ji 3 iq; f fl';r - f fl';r i Ji 3 iq; ~ -~ i Ji ~ - ~ q ;r ~ # l iT, J 3 1 - & 175" : rm- i f m # I f f ~ q r<it J iq;T 3 ' Jft c arrying q uestions, c arrying q uestions, a nswer s hort v ery a re 5 t o 1 n umber D . a nd C B , A , - s ections f our c ontains p aper Q uestion e stions. q one of the choices in :rTrr " fit J f !T q ~ you , 4 ~ f fj;r a nswer s hort a re 1 5 t o 6 e ach. uestions f ive o f q uestions t wo a nd m arks t hree o f q uestion o ne i n o vided p ere is no overall choice. However, aT:.internal ~ ~ I However, q i t ~ f f ; rtff answer 3 i f ~ - ~ - short 11 i t F \iRif ~ '31rnC1~rt<7r/.{q, # F \iRif m I f f ~ are also ~ F F \iRif ~ m ~ ) ~ 3 #<7 is not permitted. \iRif ~ ~ ~ q , 1 ftff(t i f m f uestions number 26 to 28 are long 5 marks each. ' 1 r t.{ i t;rr : J"fr( " fft q ;r ' c:T n umber m ark 1 only Jft, f < r t ~ C 1 ( 9~rt</r/.{q, m m I ~ . 3 1Rqrc/ uestions number 16 to 25 c rrying 3 marks each. fj;r G 'W-6rt</r/.{q, ' Jft m i Ji ( . ~ - ~ m uestions to attempt I f f q f;:frrr m i Ji i Ji ~ q r<it J iq;T 5 ~ ~ T e of calculators ~ ~ i Ji ~ - ~ ~ if ~ You have ~ - " # 15 (fit; " (fit; it 5 q;( " (fit; " (fit; 6 2 1 5 # I ~ . k "ft( !ff 6 "~" ff 1 ~ 1 t 'P "ft( !ff < ~ " 2 marks iT J ~ arks. ~ q " ;r - , til<fV,4/' ' - ~ i" ' ! : ~ : ;;, i i, "' c. ?ic :,c i' " ~ ' fi -- ~ "., "f!{' _i "i! , II II questions are compulsory. choice has been such each. answer questions, questions, carrying may use log t bles, if necessary. ~~~I I , ' .,. I 2 I u ses. t heir a nd d atabases B ioinformatics t wo a ny I ndicate ~GT~~.;g~~~~~~m~~~~ 7 . triphosphate and its role in ~ ~ ~ ~ ~ ur D NA ~ ~ ~.lfIT~ ~~~I ~31~~31l~ ~ 2 of a dideoxynucleotide s the structure equencing. Write DNA 8. 2 ? ~ ~ ' qRT ~ ~ q ,1 Why is 'curd' considered beneficial? '~' 9. 10. Briefly list the features of finite cell lines and continuous cell lines. ~q,1fu"q)T~~~qj)ftm~~~~~~~ somaclones in microbial ~ MI-.1I(.Y1lo1:g"(1 ~ ( ~ d) ( ( b)~, ( qm-;:r), ~ 13. Genome analysis has the potential patients ~ q ffi W r-~I~m:ift ~ ~ ~ m -m ~ ~ ~ 2 e nzyme I t he ~ i n o perate s ystem r elay c harge ~ 2 c' ? ~ W ~ " 5I"qjR ~ 4 ~ ~ ~ 99 ~ 2 ~ Chymotrypsin? ~1oi1~1~ with disease ~1oi~1~~ I I ~ ~ ~ to identify fu~~~~~_~~~~, t he d oes H ow 15. : the principle of 'insertional inactivation'. '~-R~' 14. Highlight liquor. Explain. ~ " ;:iTT m ~!fur 1 -j~T.jI-iT q,1 ~ susceptibilities. (d) Corn-steep 2 I (c) Antifoams, cell cultures: ~ (b) Agar, ~ftm ~ ~ (a) I the use of the following (a) Aeration, ~~~ ~ ~ Indicate ~ c) 12. ~ 2 ~ ~1ct"<:1r~ ~c.I~ ~ and gametoclones. -~ between ~ Differentiate I ~, 11. 2 " "-""~ ~1'~]1.~.',=~ SECTION A grobacterium u sing a ' 5!Tt{i ~ ~ ' 5I"q:jR ~ ~ ~ I ~ , ~ ~ -~ ~ m UT ~ ~ ? ~ " ;JImT 3 steps. ~ salient ~j;{I,.s(c;c7n4J-{ J-f7ij;rn4.-.1;{"q)T ~ ~ chieved t issue p the ~ ? Indicate ~ tumefaciens lant o f t ransformation i s H ow 16. ~~ C 17. Outline the steps and principles involved in isolating Streptomycin, an extracellular microbial product. ~~~ ~ ~ ~W~~ ~-~fu~~~, 18. Schematically depict Hybridization "tRja~ mUT 31ffi ~ "ij?-1T~ ~'-<.I~I~I~'i~ ~~ct<:h(UI ~-~ -q I ~fiyq)T-~ 3 the steps involved in Fluorescence In Situ 3 (FISH). ~ ~ QI~~,sI~~~I'i (FISH) -q ~ ~ ";q"{uTf ~ qj) ~ ~ ~~-q~~1 3 1 R a ~ 1ft{g ~ ~ I f qfq ~ ~ m ethods? I ~ ~ ~ c 3 - ulturing of ? ~ the principle 1rrrrrr.rt uTrqj(1f t han ~ ~ P CR-~ f qfq ~ e ffective m ore m ~ 3 w hich capacity t a s sub-units by of their i beta binding d oxygen or s alpha I s ~ ub-unit -q etermined b i t c 3 - q ~ a ~ a m "\jffift ~ a :j1C!~~~dl m ~ ~ 1~1.<.j cst~ ~ " ~ 3 ""q"-~ ~ ~ \m ~fiyq)Tan 311 I \11 ~ ~ 'i ;JI) 3 ""q"-~~ W " ij?-1T ~ ~ ~ m ' ~ \ifT m r R explain include excess? ~~ ~ methods -q ~~~I~~ ~~ excess impaired -q QI~I.~I~'i ~ ~ ~ to ~~ a:jJ5I~ produce an H ~~~II~~I ~ ~~ ~I ~ ~ - q patients ow in Newer and its applications. am ~ leading erythrocytes. produced ~ fu~ e Thalassaemic haemoglobin i 3 1fqqj ~ ~ P - q ~ CR-~ culture \111C!~~~ (}fIc.I'-<1I~) ~ 21. t i s ~ ~ H ow ~ " ;J{ - I ~ q, a ssay. ~ ~ " U1T ~ an ~ ~ 31;rqftR Describe protoplast sputum. the help of a diagram m 20. ~ ~QI~dl fqf~ ~ ~ ~ PCR-based PCR-based assays. With from depended on ~ bacillus o causative the older methods ~ the ~ culturing of tuberculosis, ~ 19. In the diagnosis ~if~~~? ~'-' 99 5 P.T.D. Which 3 s uch t hree I a tleast N ame ~ m ethods. q ~ - ;rrJ:r t ~ mm m o;:r ~ -~-~ ~ ~ ? ~ ~ method is best suited for obtaining maximum c oli. E . i n i ntracellularly e xpressed i s p rotein culturing yield of Explain. 3 OR this protein? uf;:r ' q:jT t ransfer i mportant? ~ D NA m ~ d atabases 3 I l =I~1Uf ~ r ~ ~ uses. ecombinant g ~ ~ and their iven A 24. "(f2:fr ~ databases o;:r v ectorless t hree D s a re Why equence NA D escribe ~-~ 2 2. 23. ~1r1i('1~ul ' 5tTi\';:r ~ I I ~ ~ 3 ~~I~l!. ~ ? m culture. ~ ~ ~ ~ microbial ~ ~ ~ ~ 3 continuous :f('1:~~lctll~ and ~ m iTo;:r ~ ~ ~ fed-batch ~;ft ~ R f?J! ~ ~ ~ 3 c oli between =1~ ~ E. Differentiate t v irus o f w eight a 3 (c) No. of virus = cells in culture particles per animal for oxygen and CO2) 1: = 50 cell ) , ; , - n m) 1 m illion) ( 1 r 6 adius 1 06 o f = v irus s phere a o f i s m ass v irus M 99 (Assume olecular .".. I [ No. of animal be kept 1 20% space must (b) = 2000 volume j bioreactor/fermentor 05/mL Total (atleast (d) and L harvested: (a) he g rowing b the virus Based on the data nd v olume p acked t c y m ade I i s ~ v accine ~ ( FMDV) V ~ the cells, harvesting it before vaccine formulation. alculate b elow, inactivating given finally cells, breaking he virus in animal irus D isease M outh a -a-~ 3:fFq{;J ~~~~ nd F oot 25. (j:{fuf ~ ~ 3T~ - c- , ~~~~~~~; " ~ cllllllll..-==l.11 , ~ . c."c ,;! I ~ -~ \ TlT ~ ( FMDV) ~ ~ - GAR # ~ q ;) m uft ~ ~an ~ ~ ~ ~ ~ ~ , ~ man q ;) ~ ~ ~ , ~ q ;) ~ ~ ~ a W m3tffff;~~~~~ a W ~ ~~~~"qjT~~ ~~ I ~ ~"\"jfTW~'Q\~~ ~~~q;1"~aW m q -)jT;r " qjT Y R C j) ( 1 o -j ~ : ~ = 2 000 L (q;) ~ I~IR~~~~ q;1" ' c'. I (~-~-~ 2 0% ~ 3 't1~I'J1o-j ~ C O2 ~ ~ ~ ~ ~ ~ ) 1 05/mL # muft;)~an ;1" = q q~ ("&)~ (1T) : srfu m uft ~ ~ ~ ~ ~ ~ q ;1 ~ = 5 0 (~) ~ q ;1" ~ ~ = 1 06 ( 10 ~ ) Ii ( ~~~f(f)~~~~~~ 1 nm~) l I D SECTION ~G 26. W hat a re t he a dvantages o f w hole g enome s equencing p rojects? H ow i s gene p rediction c arried o ut i n s uch p rojects u sing c omputational t ools? 5 OR What i s m eant b y t he t erm " genomics" ? D ifferentiate b etween s tructural a nd f unctional g enomics. 5 . ~ ~ ~ ~ Y R~I'J1o-jlan ~ ~ ~ ~ ? Y RCj)(1o-j ~ " qjT ~ ~ ~ YR~I'J1o-jl~ # ~ ' \icf~UJT ~ ' Sfq)R q ;1" ~ ~ ? ~ "\11lo-jll~~" ~ ~ ~ ~ ~ ? ~ ~"t:Io-jl("4-fCj)"('f"ifr C j)1~f("4-fCj) \ 11lo-jll~~ # ~ ~I 27. O ne o f t he f irst e xamples o f m olecular d isease w as s ickle c ell a naemia. Describe t he t echnique w hich w as u sed t o e stablish t his d 5 iscovery. ~ \ TlT ~ ~ ~ ~ Tf # ~ ~ ~ ~ -~~ ~ I ~ ~ ~ ~ m # - q;-m 3 1rif ~ "q;j~ " qjT q uR ~ I P.T.D. 7 99 I .~ - ~--~ - ~ 5 5 ~ R FLP # u ses? ~ i ts ~ a re q j) w hat 3 :j~~ a nd D NA ~ g enerated ~ 3T~ I ~ l ibrary ~ ~ c DNA a 3 ' 3'~ 1ft& i s ~ ' }l'q:;T'{ ~ ~ How OR s equences. D NA W ith differentiating 2 8. a suitable iagram, xplain owRFLPtechnique useful. d e h is for ~ '}l'q:;T'{ ~ ffi ~ ? m2f , ~ m ~-~:am ~, ~ CI:fi ~I cDNA ~ :i:: ,'. " 99 . '!;. ~~ " ","Cc.. " 'c 8 3 700 '

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