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CBSE Class XII 2014 : BIOTECHNOLOGY

8 pages, 60 questions, 1 questions with responses, 1 total responses,

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H$moS> Z . Series OSR Code No. amob Z . 99 narjmWu H$moS >H$mo C ma-nwp VH$m Ho$ _wI-n >na Ad ` {bIo & Roll No. Candidates must write the Code on the title page of the answer-book. H $n`m Om M H$a b| {H$ Bg Z-n _o _w{ V n > 8 h & Z-n _| Xm{hZo hmW H$s Amoa {XE JE H$moS >Z ~a H$mo N>m C ma-nwp VH$m Ho$ _wI-n > na {bI| & H $n`m Om M H$a b| {H$ Bg Z-n _| >28 Z h & H $n`m Z H$m C ma {bIZm ew $ H$aZo go nhbo, Z H$m H $_m H$ Ad ` {bI| & Bg Z-n H$mo n T>Zo Ho$ {bE 15 {_ZQ >H$m g_` {X`m J`m h & Z-n H$m {dVaU nydm _| 10.15 ~Oo {H$`m OmEJm & 10.15 ~Oo go 10.30 ~Oo VH$ N>m Ho$db Z-n H$mo n T>|Jo Am a Bg Ad{Y Ho$ Xm amZ do C ma-nwp VH$m na H$moB C ma Zht {bI|Jo & Please check that this question paper contains 8 printed pages. Code number given on the right hand side of the question paper should be written on the title page of the answer-book by the candidate. Please check that this question paper contains 28 questions. Please write down the Serial Number of the question before attempting it. 15 minutes time has been allotted to read this question paper. The question paper will be distributed at 10.15 a.m. From 10.15 a.m. to 10.30 a.m., the students will read the question paper only and will not write any answer on the answer-book during this period. O d- m mo{JH$s BIO-TECHNOLOGY {ZYm [aV g_` : 3 K Q>o A{YH$V_ A H$ : 70 Time allowed : 3 hours 99 Maximum Marks : 70 1 P.T.O. gm_m ` {ZX}e : (i) g^r Z A{Zdm` h & (ii) H$moB g_J M`Z-{dH$ n (AmodaAm b Mm Bg) Cnb Y Zht h & {\$a ^r, 3 A H$m| dmbo EH$ Z _| VWm 5 A H$m| dmbo Xmo Zm| _| ^rVar M`Z-{dH$ n Cnb Y h & Eogo Zm| _| AmnH$mo Ho$db EH$-EH$ {dH$ n H$m hr C ma XoZm h & Z-n _| Mma I S> A, ~, g VWm X h & (iii) Z-g `m A H$ h & (iv) Z-g `m 6 go 15 VH$ Ho$ Z bKy mam _H$ h , {OZ_| go `oH$ Ho$ Xmo-Xmo A H$ h & (v) Z-g `m h & (vi) Z-g `m 26 go 28 VH$ Ho$ Z XrK -C mam _H$ h , {OZ_| go `oH$ Ho$ nm M-nm M A H$ h & (vii) H $bHw$boQ>am| (JUH$m|) H$m Cn`moJ d{O V h & {\$a ^r, `{X Amd `H$ hmo, Vmo Amn bm J-gma{U`m| H$m Cn`moJ H$a gH$Vo h & 1 go 16 go 5 VH$ Ho$ Z A{VbKy mam _H$ Z h , {OZ_| go `oH$ H$m EH$-EH$ 25 VH$ Ho$ Z ^r bKy mam _H$ h , {OZ_| go `oH$ Ho$ VrZ-VrZ A H$ General Instructions : (i) All questions are compulsory. (ii) There is no overall choice. However, an internal choice has been provided in one question of three marks and two questions of five marks. You have to attempt only one of the choices in such questions. Question paper contains four sections A, B, C and D. (iii) Questions No. 1 to 5 are very short answer questions, carrying 1 mark each. (iv) Questions No. 6 to 15 are short answer questions, carrying 2 marks each. (v) Questions No. 16 to 25 are also short answer questions, carrying 3 marks each. (vi) Questions No. 26 to 28 are long answer questions, carrying 5 marks each. (vii) Use of calculators is not permitted. However, you may use log tables, if necessary. 99 2 I S> A SECTION A 1. _mZd OrZmo_ _| Cggo X JwZr g `m _| OrZ hmoVo h {OVZo {H$ EH$ gab Ord S >mogmo{\$bm _ob ZmoJo Q>a _| hmoVo h & n >rH$aU H$s{OE {H$ Bggo _m XwJwZr g `m Ho$ OrZ CVZr O{Q>bVm XmZ H$a gH$Vo h , {OVZr H$s _mZdm| _| hmoVr h & 1 The Human Genome contains twice as many genes as the simple organism Drosophila melanogaster. Give one explanation as to how just double the number of genes can give rise to the complexity required for a human being. 2. N>moQ>o n _mZo na `moJembm g dY Z H$aZo _| g p b > _m `_ gabVa `m| hmoZm Mm{hE ? For small scale laboratory culturing, why is synthetic media simpler ? 1 3. in vitro (nm o) VWm in vivo (Ordo) e Xm| _| {d^oX H$s{OE & Differentiate between the terms in vitro and in vivo . 1 4. `moJembm Ho$ ^rVa N>moQ>o n _mZo na _m `_ g dY Z Ho$ {bE H$m Zgm {ZO _uH$aU H $_U Cn`moJ {H$`m OmVm h ? 1 What is the sterilization procedure used for small scale media culturing in laboratory ? 5. EH$ CXmhaU XoVo h E mW{_H$ H$mo{eH$m g dY Zm| H$s n[a^mfm Xr{OE & 1 With an example define primary cell cultures. I S> ~ SECTION B 6. H$V Z (aop Q > eZ) E OmB_m| H$mo `h Zm_ `m| {X`m J`m BZH$m `m _h d h ? ? nwZ`m}JO DNA m mo{JH$s _| 2 Why are restriction enzymes so named ? What is their importance in recombinant DNA technology ? 7. A ` ~ hX AUwAm| go {^ Vm Xem Vo h E, moQ>rZm| _| ^mar {d{dYVm hmoVr XoIr OmVr h & Eogm `m| ? {H$ ht Xmo moQ>rZm| Ho$ Zm_ {b{IE Am a CZHo$ H$m` ~VmBE & 2 Unlike other macromolecules, proteins show enormous diversity. Why ? Name any two proteins and their function. 99 3 P.T.O. 8. OrZmo_ AZwH $_U n[a`moOZmAm| H$mo `m`mo{MV R>hamZo Ho$ nj _| Xmo H$maU ~VmBE & 2 Give two reasons to justify genome sequencing projects. 9. gy _O {dH$ g dY Z _| B Vo_mb {H$E OmZo dmbo Xmo H$m M Ho$ YmaH$ nm m| Ho$ Zm_ {b{IE & BZHo$ ^rVa dmVZ H$mo {H$g H$ma C V {H$`m OmVm h ? 2 Name two glass holding vessels used in microbial culture. How is aeration improved in these ? 10. nmXn H$mo{eH$mAm| H$m dh _h dnyU JwUY_ ~VmBE {OgHo$ H$maU nmXn D$VH$ g dY Z hmo nmVm h & nmXn D$VH$ g dY Z H$m H$moB EH$ AZw `moJ gwPmBE & 2 Name the important property of plant cells that enables plant tissue culture. Suggest any one application of plant tissue culture. 11. Eogm `m| h {H$ gra_ mUr H$mo{eH$m g dY Z H$m EH$ _h dnyU g KQ>H$ hmoVm h ? 2 Why is serum an important constituent of animal cell culture ? 12. Eogo Xmo d km{ZH$m| Ho$ Zm_ {b{IE Ed CZH$m dh `moJXmZ ~VmBE Omo C hm|Zo moQ>rZ g aMZm Ho$ n >rH$aU _| {X`m h & 2 Name two scientists with their contribution in the elucidation of protein structure. 13. EH$ `moOZm AmaoI H$s ghm`Vm go ~VmBE {H$ {H$g H$ma go H$moB Am fY Ho$ {bE A{YH$ ^m ` hmo gH$Vm h & SNP {M U dmbm {H$gr 2 With the aid of a schematic diagram indicate how a certain SNP profile is more susceptible to a drug. 14. dh {H$g $n dmbm g dY , A J AWdm ~{h amon g dY hmoVm h {Ogo H $bg {Z_m U o[aV H$aZo _| Am_Vm a go B Vo_mb {H$`m OmVm h ? H $bg D$VH$ Cn`moJr `m| hmoVm h ? Which type of culture, organ or explant culture, is generally used to induce callus formation ? Why is callus tissue useful ? 99 4 2 15. ZrMo Xr Om ahr Vm{bH$m _| Xmo OrdYm[a`m|, `r Q> VWm _mZd Ho$ {df` _| OrZmo_ gmB O VWm OrZ ^{d `gyMZmE Xr Om ahr h & BZgo Amn H$m Z-go Xmo ojU m H$a gH$Vo h , {b{IE & Ord `r Q> _mZd 2 H $mo_mogmo_ g `m OrZmo_ gmB O (bp) OrZm| H$s g `m 16 23 12,068,000 3,000,000,000 6,340 25,000 Given below is a table of genome size and gene predictions for two organisms, yeast and human. Indicate two observations you can make. Organism No. of chromosomes Genome size (bp) No. of genes Yeast 16 12,068,000 6,340 Human 23 3,000,000,000 25,000 I S> g SECTION C 16. moQ>rZ Ho$ ^rVa Aghg `moOr Am~ Ym| Ho$ VrZ $nm| na g {j {Q> n{U`m {b{IE & 3 Write short notes on three types of non-covalent bonds in a protein. 17. do Q>a (dmhH$) `m hmoVm h ? ~ Q>r[a`b do Q>a Ho$ VrZ _h dnyU bjU {JZmBE & 3 What is a vector ? Enumerate three important features of a bacterial vector. 18. OrZmo_ Ho$ H$moS>ZH$mar VWm AH$moS>ZH$mar ^mJm| Ho$ ~rM nmE OmZo dmbo A Va g_PmBE & {H$gr EH$ Eogo SNP H$m CXmhaU Xr{OE {Oggo AIDS {VamoY n Xm hmo OmVm h & 3 Explain the differences between coding and non-coding parts of a genome. Give an example of a SNP which leads to AIDS resistance. 19. EH$ J m \$ H$s ghm`Vm go Q>{~ S>mo Q> Q> (Yw Yb Wm`r) VWm H$s_mo Q> Q> (agmo Wm`r) _| {d^oX H$s{OE Am a g_PmBE {H$ H$moB Q>oS>r- Q>oQ> (p Wa- Wm`r) `m hmoVr h & 3 Differentiate between a turbidostat and chemostat. With the help of a graph, explain what a steady-state is. 99 5 P.T.O. 20. dm`ag-_w $ nm Yo _h dnyU `m| hmoVo h ~Zm`m OmVm h , g jon _| dU Z H$s{OE & ? ~ S>o n _mZo na H ${f Ho$ {bE B h| {H$g H$ma 3 Why are virus-free plants important ? Briefly describe how these are made for large scale cultivation. 21. g jon _| g_PmBE {H$ mUr H$mo{eH$m g dY Z _| h & CO2 BZ `y~oQ>am| H$s Amd `H$Vm `m| hmoVr 3 Explain why CO2 incubators are required for animal cell culture. 22. DNA AZwH $_U H$s gm Joa {d{Y H$m _w ` {g m V `m h , g jon _| g_PmBE & dMm{bV DNA AZwH $_U _| Cn`moJ H$aZo hoVw ddNTPs _| `m- `m $nm VaU {H$E OmVo h ? 3 Briefly explain the main principle of the Sanger method of DNA sequencing. What modifications are done in ddNTPs for using them in automated DNA sequencing ? 23. EH$ Cn`w $ CXmhaU XoVo h E ~VmBE {H$ {H$gr gy _Ordr` ^oX H$mo {H$gr EH$ AmYw{ZH$ O d- m mo{JH$s {d{Y mam {H$g H$ma Am a ~ohVa ~Zm`m Om gH$Vm h & 3 AWdm ~ Q>r[a`m _| gwH|$ H$s` OrZm| H$s A{^ `{ $ H$aZo _| AmZo dmbr Xmo g_ `mE `m - `m h , ~VmBE & BZ na nma nmZo _| `r Q> H$mo{eH$mAm| H$m {H$g H$ma Cn`moJ {H$`m Om gH$Vm h ? 3 With a suitable example indicate how a microbial strain can be improved by any one modern biotechnological method. OR Indicate two problems which are encountered in expressing eukaryotic genes in bacteria. How can yeast cells be used to overcome these ? 24. mUr H$mo{eH$mAm| Ho$ ^rVa OrZ doe H$amZo H$s VrZ {d{Y`m ~VmBE Am a gmW hr `oH$ {d{Y _| {Z{hV {g m V ^r g jon _| ~VmBE & `{X {H$gr OrZ {M{H$ gm `moJ _| OrZm| H$mo nh MmZo Ho$ {bE {H$gr OmBJmoQ> (`w _ZO) H$m Cn`moJ H$aZm hmo , Vmo ~VmBE H$m Z-gr doe {d{Y ~ohVa hmoJr & Mention three methods of gene delivery into animal cells with a brief explanation of their principles. If a zygote has to be used for delivering genes in a gene therapy experiment, which mode of delivery would be preferable ? 99 6 3 25. Q>o_ gob m mo{JH$s ^{d ` Ho$ {bE C moOZerb g ^mdZmE XmZ H$aVr h & a $mo n{ m H$mo EH$ AZw `moJ Ho$ $n _| boVo h E Bg ZB m mo{JH$s VWm BgHo$ Cn`moJ Ho$ {df` _| g_PmBE & 3 Stem cell technology offers exciting possibilities for the future. Using haematopoiesis as an application, explain this new technology and its use. I S> X SECTION D 26. Xm r H$mo{eH$m Aa $Vm EH$ {d d gH$mar amoJ h Omo Hw$N> Am~m{X`m| H$mo ^m{dV H$a ahm h & Bg amoJ _| {Z{hV Amp dH$ {H $`m{d{Y Ho$ {df` _| g_PmBE Am a EH$ Eogr {d{Y H$m dU Z H$s{OE {OgHo$ mam Bgo `moJembm Ho$ ^rVa nhMmZm Om gH$Vm h & 5 AWdm g_PmBE {H$ E OmB_ H$mB_mo{Q >p gZ Ho$ ghr db`Z go hr dh EH$ moQ>rZb`H$ E OmB_ Ho$ $n _| H$m` H$a gH$Vm h & Bgr {H $`m{d{Y H$m Cn`moJ H$aZo dmbo Xmo A ` E OmB_m| Ho$ Zm_ {b{IE & 5 Sickle cell anaemia is a devastating disease affecting some populations. Explain the molecular mechanism involved in this disease and describe a method to identify it in the laboratory. OR Explain how the correct folding of the enzyme chymotrypsin leads to its function as a proteolytic enzyme. Name two more enzymes which use the same mechanism. 99 7 P.T.O. 27. EH$ AmaoI XoVo h E ~VmBE {H$ gXZ g H$aU VH$ZrH$ H$m {g m V `m h & {H$gr OrZmo_ _| {H$gr EH$ {deof OrZ Ho$ _m OyX hmoZo Ho$ g gyMZ _| Bg {g m V H$m AZw `moJ ~VmBE & 5 AWdm DNA bmB~ oar ~ZmZo _| `m- `m MaU AmVo h , EH$ Zm_m {H$V AmaoI XoVo h E ~VmBE & OrZmo_r DNA bmB~ oar bm^H$mar `m| hmoVr h ? 5 Indicate the principle of the Southern hybridisation technique with a diagram. Apply this principle to detect the presence of a particular gene in a genome. OR With a labelled diagram, indicate the steps to make a DNA library. Why is a genomic DNA library useful ? 28. AZw dmh H $_U `m hmoVm h ? do VrZ _h dnyU XemE ~VmBE Omo BgHo$ {bE Amd `H$ hmoVr h & H$moB EH$ Eogr VH$ZrH$ g_PmBE {OgHo$ mam {H$gr ew{ H $V moQ>rZ H$m bjUZ {H$`m OmVm h & 5 What is downstream processing ? List three important conditions to be observed for the same. Explain any one technique used to characterize a purified protein. 99 8 4,100

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